Cao Yunpeng, Li Shumei, Han Yahui, Meng Dandan, Jiao Chunyan, Abdullah Muhammad, Li Dahui, Jin Qing, Lin Yi, Cai Yongping
School of Life Sciences, Anhui Agricultural University, Hefei 230036, People's Republic of China.
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, People's Republic of China.
R Soc Open Sci. 2018 Jul 18;5(7):171463. doi: 10.1098/rsos.171463. eCollection 2018 Jul.
In plants, plant fructokinases (FRKs) are considered to be the main gateway of fructose metabolism as they can phosphorylate fructose to fructose-6-phosphate. Chinese white pears () are one of the popular fruits in the world market; sugar content is an important factor affecting the quality of the fruit. We identified 49 from four Rosaceae species; 20 of these sequences were from Chinese white pear. Subsequently, phylogenic relationship, gene structure and micro-collinearity were analysed. Phylogenetic and exon-intron analysis classified these s into 10 subfamilies, and it was aimed to further reveal the variation of the gene structure and the evolutionary relationship of this gene family. Remarkably, gene expression patterns in different tissues or different development stages of the pear fruit suggested functional redundancy for derived from segmental duplication or genome-wide duplication and sub-functionalization for some of them. Additionally, , and were found to play important roles in regulating the sugar content in the fruit. Overall, this study provided important insights into the evolution of the gene family in four Rosaceae species, and highlighted its roles in both pear tissue and fruits. Results presented here provide the appropriate candidate of s that might contribute to fructose efflux in the pear fruit.
在植物中，植物果糖激酶（FRKs）被认为是果糖代谢的主要途径，因为它们可以将果糖磷酸化为6-磷酸果糖。中国白梨（）是世界市场上受欢迎的水果之一；糖分含量是影响果实品质的重要因素。我们从四个蔷薇科物种中鉴定出49个；其中20个序列来自中国白梨。随后，分析了系统发育关系、基因结构和微观共线性。系统发育和外显子-内含子分析将这些基因分为10个亚家族，旨在进一步揭示该基因家族的基因结构变异和进化关系。值得注意的是，梨果实不同组织或不同发育阶段的基因表达模式表明，由片段重复或全基因组重复产生的基因存在功能冗余，其中一些基因存在亚功能化。此外，发现、和在调节果实糖分含量方面发挥重要作用。总体而言，本研究为四个蔷薇科物种中基因家族的进化提供了重要见解，并突出了其在梨组织和果实中的作用。这里给出的结果提供了可能有助于梨果实中果糖外排的合适基因候选物。
