Hakim Hakimullah, Toyofuku Chiharu, Ota Mari, Suzuki Mayuko, Komura Miyuki, Yamada Masashi, Alam Md Shahin, Sangsriratanakul Natthanan, Shoham Dany, Takehara Kazuaki
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8, Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
J Vet Med Sci. 2017 Feb 4;79(2):244-247. doi: 10.1292/jvms.16-0553. Epub 2016 Nov 25.
An alkaline agent, namely food additive grade calcium hydroxide (FdCa (OH)) in the powder form, was evaluated for its bactericidal efficacies in chicken feces at pH 13. The point for this evaluation was neutralization of the alkaline agent's pH at the time of bacterial recovery, since otherwise the results are substantially misleading. Without neutralization of the FdCa (OH) pH, the spiked bacteria were killed within min at the time of recovery in aqueous phase, but not in the solid form in feces, hence, it has been demonstrated that when bacteria were in solid, it took longer time than in liquid for the alkaline agent to inactivate them down to the acceptable level (≥3 log CFU/ml).
对一种碱性试剂，即粉末状食品添加剂级氢氧化钙（FdCa(OH)）在pH值为13的鸡粪中的杀菌效果进行了评估。此次评估的关键在于在细菌复苏时中和碱性试剂的pH值，因为否则结果会产生极大误导。如果不中和FdCa(OH)的pH值，在水相中复苏时，接种的细菌在数分钟内就会被杀死，但在粪便的固体形态中则不会，因此，已经证明当细菌处于固体状态时，碱性试剂将其灭活至可接受水平（≥3 log CFU/ml）所需的时间比处于液体状态时更长。
