Doorbar J, Campbell D, Grand R J, Gallimore P H
EMBO J. 1986 Feb;5(2):355-62. doi: 10.1002/j.1460-2075.1986.tb04219.x.
Antibodies prepared against a human papilloma virus-1 (HPV-1) E4/beta-galactosidase fusion protein identified several polypeptides in HPV-1, but not HPV-2 or 4, induced papillomas. The major E4 protein, that represented up to 30% of total cellular protein, was a 16/17-K doublet which was purified by column chromatography and analysed for amino acid content. A peptide derived by chymotryptic digestion was purified by h.p.l.c. and subjected to amino acid sequencing. The unique sequence obtained, Gly-His-Pro-Asp-Leu-Ser-Leu, identified the 16/17-K doublet as a product of the HPV-1 E4 gene region. Antibodies to both the E4/beta-galactosidase fusion protein and the 16/17-K doublet identified two smaller polypeptides (10/11-K) which may represent spliced products of E4. We propose that the products of the HPV-1 E4 gene region are not classical DNA tumor virus early proteins and suggest that they play a role in virus maturation.
针对人乳头瘤病毒1型（HPV-1）E4/β-半乳糖苷酶融合蛋白制备的抗体，在HPV-1诱导的乳头瘤中鉴定出了几种多肽，但在HPV-2或4诱导的乳头瘤中未鉴定出。主要的E4蛋白占细胞总蛋白的30%，是一种16/17-K的双峰蛋白，通过柱层析法纯化，并对其氨基酸含量进行了分析。通过胰凝乳蛋白酶消化得到的一种肽经高效液相色谱法纯化后进行氨基酸测序。所获得的独特序列Gly-His-Pro-Asp-Leu-Ser-Leu，确定16/17-K双峰蛋白是HPV-1 E4基因区域的产物。针对E4/β-半乳糖苷酶融合蛋白和16/17-K双峰蛋白的抗体鉴定出了两种较小的多肽（10/11-K），它们可能是E4的剪接产物。我们提出，HPV-1 E4基因区域的产物不是典型的DNA肿瘤病毒早期蛋白，并表明它们在病毒成熟过程中发挥作用。
