Gertman E, White B N, Berry D, Kropinski A M
J Bacteriol. 1986 Jun;166(3):1134-6. doi: 10.1128/jb.166.3.1134-1136.1986.
A new insertion element, IS222, was identified to be associated with the DNA of a mutant strain of the converting Pseudomonas aeruginosa bacteriophage D3. The insertion sequence was 1,350 base pairs in size and possessed terminal inverted repeats. The nucleotide sequence contained single cleavage sites for EcoRI and PvuI but none for BamHI, PstI, HindIII, SmaI, or SalI. By Southern hybridization analysis, no homology was found with genomic DNA from P. aeruginosa PAT or Escherichia coli. Genomic DNA from the phage host, P. aeruginosa PAO, contained two sequences homologous to IS222.
一种新的插入元件IS222被确定与转化型铜绿假单胞菌噬菌体D3突变株的DNA相关。该插入序列大小为1350个碱基对，并具有末端反向重复序列。核苷酸序列含有EcoRI和PvuI的单一切割位点，但没有BamHI、PstI、HindIII、SmaI或SalI的切割位点。通过Southern杂交分析，未发现与铜绿假单胞菌PAT或大肠杆菌的基因组DNA有同源性。噬菌体宿主铜绿假单胞菌PAO的基因组DNA含有两个与IS222同源的序列。
