Institution of Neurology of Jiangxi Province, Department of Neurology, People's Hospital of Jiangxi Province, Nanchang 330006, Jiangxi Province, China.
Institution of Neurology of Jiangxi Province, Department of Neurology, People's Hospital of Jiangxi Province, Nanchang 330006, Jiangxi Province, China.
Biomed Pharmacother. 2018 Oct;106:1236-1242. doi: 10.1016/j.biopha.2018.07.058. Epub 2018 Jul 20.
Parkinson's disease (PD) is caused by the gradual loss of dopamine-producing cells in the brain. This study evaluated the potential neuroprotective role of puerarin (PR) on dopamine (DA)-producing cells in vitro and in vivo.
In vitro, the effects of PR on proliferation and differentiation and DA releases of mesenchymal stem cells (MSCs) were assayed by CCK-8, flow cytometry, real-time PCR and ELISA respectively. Then the differentiated cells were labeled with enhanced green fluorescent protein (EGFP) and administrated into PD animal models induced by 6-OHDA. The proliferation and differentiation of labeled cells were identified by fluorescence microscopy and immunostaining.
In vitro, after being treated with different concentrations of PR for 1 week, the TUJ1, TH and DAT protein and mRNA expression and DA releases increased significantly. In vivo, after transplantation of PR-treated DA-producing cells, the symptoms of PD improved significantly from the second week after transplantation; more transplanted cells survived and migrated to wider region along injection line; more transplanted cells proliferated and differentiated into TH cells; more DA was detected in the striatum during 6 weeks' observation.
The results suggest that PR promote DA neuron survival, proliferation and differentiation.
帕金森病（PD）是由大脑中产生多巴胺的细胞逐渐丧失引起的。本研究评估了葛根素（PR）对体外和体内多巴胺（DA）产生细胞的潜在神经保护作用。
在体外，通过 CCK-8、流式细胞术、实时 PCR 和 ELISA 分别检测 PR 对间充质干细胞（MSCs）增殖、分化和 DA 释放的影响。然后将分化的细胞用增强型绿色荧光蛋白（EGFP）标记，并给予 6-OHDA 诱导的 PD 动物模型。通过荧光显微镜和免疫染色鉴定标记细胞的增殖和分化。
在体外，用不同浓度的 PR 处理 1 周后，TUJ1、TH 和 DAT 蛋白和 mRNA 表达及 DA 释放明显增加。在体内，PR 处理后的 DA 产生细胞移植后，从移植后第 2 周开始，PD 症状明显改善；更多的移植细胞存活并沿着注射线迁移到更宽的区域；更多的移植细胞增殖并分化为 TH 细胞；在 6 周的观察期间，纹状体中检测到更多的 DA。
这些结果表明，PR 促进 DA 神经元的存活、增殖和分化。
