1 State University of Maringá, Maringá, Paraná, Brazil.
2 Sperm Analysis Section, São Camilo Laboratory, Maringá, Paraná, Brazil.
Am J Mens Health. 2018 Nov;12(6):2104-2115. doi: 10.1177/1557988318794522. Epub 2018 Aug 22.
To access the possibility that key markers of bacterial vaginosis (KM-BV) could affect seminal parameters and thus fertility a prospective cohort study was designed (a) to develop rapid and sensitive multiplex polymerase chain reaction (M-PCR) assays to screen 13 key markers of bacterial vaginosis (KM-BV) in semen specimens, (b) to determine the prevalence of KM-BV in semen from randomized male partners of couples seeking fertility evaluation. A total of 229 semen samples were included in the study from males who visited the Sperm Analysis Section of Brazil between October 2015 and March 2016. Eligible men were 18 years or older and had a semen analysis due fertility evaluation (after failing to conceive with their partner after 1 year of unprotected intercourse). Basic seminal parameters were analyzed, and KM-BV was detected by M-PCR assays. M-PCR assays clearly distinguished 13 KM-BV in 146 semen samples (63.8%), mainly Gardnerella vaginalis (50.7%). Some important associations occurred between the presence of KM-BV in semen and changes in seminal parameters. KM-BV is commonly present in the semen of males seeking fertility evaluation and could potentially play significant roles in male subfertility and/or infertility.
为了研究细菌性阴道病（BV）的关键标志物（KM-BV）是否可能影响精液参数并进而影响生育能力，我们设计了一项前瞻性队列研究：（a）开发快速、敏感的多重聚合酶链反应（M-PCR）检测方法，以筛选精液标本中的 13 种关键的细菌性阴道病标志物（KM-BV）；（b）检测寻求生育评估的夫妇中随机男性伴侣精液中 KM-BV 的流行率。共有 229 份精液样本纳入了这项研究，这些样本均来自于 2015 年 10 月至 2016 年 3 月期间到巴西精子分析科就诊的男性。纳入研究的男性年龄在 18 岁或以上，且由于其伴侣在未采取避孕措施的情况下性交 1 年仍未怀孕而进行了精液分析以进行生育评估。分析了基本的精液参数，并通过 M-PCR 检测方法检测了 KM-BV。M-PCR 检测方法明确区分了 146 份精液样本中的 13 种 KM-BV，主要为阴道加德纳菌（50.7%）。KM-BV 在精液中的存在与精液参数的变化之间存在一些重要的关联。KM-BV 在寻求生育评估的男性精液中普遍存在，可能在男性生育力低下和/或不育中发挥重要作用。
