Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
Ecotoxicol Environ Saf. 2018 Nov 30;164:434-439. doi: 10.1016/j.ecoenv.2018.08.046. Epub 2018 Aug 22.
The use of potent microbial mixed cultures is a promising method for the bioremediation of recalcitrant compounds. In this study, eight molds, three yeasts, and four bacterial isolates were screened from an aged oil-polluted area. An oil degradation assay with various combinations including Bacterial Mixed Culture (BMC), Fungal Mixed Culture (FMC), Fungal-Bacterial Mixed Culture (TMC), and Sequential Fungal-Bacterial Mixed Culture (SMC) was investigated. The results indicated that the SMC culture had the highest yield of degradation (65.96%) in comparison with the degradation yields of TMC, FMC and BMC, which were 59.04%, 56.64%, and 47.56%, respectively. The degradation of saturates, aromatics, resins, and asphaltenes in the crude oil found using the Iatroscan system were, as follows: 64.21%, and 67.63% for aromatics, 72.90%, and 73.59% for saturates, and 53.88% and 58.25% for resins with respect to the TMC and SMC cultures as the superior mixed cultures. The growth rates of yeasts, molds, and bacteria in the TMC and SMC cultures were compared for further evaluation of the role of each microorganism in the degradation. Our findings support the use of mixed cultures in the bioremediation of recalcitrant petroleum pollution.
使用有效的微生物混合培养物是修复难降解化合物的一种很有前途的方法。在这项研究中，从一个老化的油污区筛选出了八种霉菌、三种酵母和四种细菌分离物。利用细菌混合培养物（BMC）、真菌混合培养物（FMC）、真菌-细菌混合培养物（TMC）和顺序真菌-细菌混合培养物（SMC）进行了各种组合的油降解实验。结果表明，SMC 培养物的降解率（65.96%）最高，与 TMC、FMC 和 BMC 的降解率（分别为 59.04%、56.64%和 47.56%）相比，TMC、FMC 和 BMC 的降解率分别为 59.04%、56.64%和 47.56%。使用 Iatroscan 系统发现，原油中饱和物、芳烃、树脂和沥青质的降解情况如下：对于芳烃，TMC 和 SMC 培养物的降解率分别为 64.21%和 67.63%；对于饱和物，TMC 和 SMC 培养物的降解率分别为 72.90%和 73.59%；对于树脂，TMC 和 SMC 培养物的降解率分别为 53.88%和 58.25%。为了进一步评估每种微生物在降解中的作用，比较了 TMC 和 SMC 培养物中酵母、霉菌和细菌的生长率。我们的研究结果支持在难生物降解石油污染的生物修复中使用混合培养物。
