Çiftci Gülay, Yiğit Özkan, Çiftci Alper
Department of Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayis, 55200 Atakum, Samsun, Turkey.
Department of Microbiology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, 55200 Atakum, Samsun, Turkey.
Trop Anim Health Prod. 2019 Feb;51(2):355-361. doi: 10.1007/s11250-018-1695-3. Epub 2018 Aug 25.
The genus Brucella causes significant economic losses due to infertility, abortion, stillbirth or weak calves, and neonatal mortality in livestock. Brucellosis is still a zoonosis of public health importance worldwide. In the past, vaccination was administered subcutaneously and nowadays, the conjunctival vaccine is administered. There is no definite information about the changes of the biochemical parameters and antibody response after conjunctival vaccination. In this study, the investigation of the changes in the levels of some biochemical parameters due to the conjunctival vaccination for brucellosis was aimed. Thirty sheep were used as an animal material. The vaccine was done single dose against Brucella melitensis and the blood was drawled from Vena jugularis during 4 months. Antibody levels were determined by serum tube agglutination test. Alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), glucose, total protein, and albumin levels were measured using commercial autoanalyzer in sera. The antibody titers (p < 0.001) increased significantly at first month compared to the pre-vaccination, but at the second month began to fall. There was no statistically significant changes in glucose, AST, ALT activity after vaccination (p > 0.005). The significant amount of total protein and ALP decreased after vaccination (p < 0.005). LDH levels and total protein levels were significantly increased (p < 0.005). In conclusion, conjunctival vaccine was considered to be used as a safe to protect the sheep from brucellosis and the results of the study may be used to improve the efficiency of brucellosis eradication programs within livestock management.
布鲁氏菌属会导致家畜不孕、流产、死产、犊牛体弱及新生仔畜死亡，从而造成重大经济损失。布鲁氏菌病在全球范围内仍是具有公共卫生重要性的人畜共患病。过去，疫苗通过皮下注射接种，如今则采用结膜疫苗接种。关于结膜疫苗接种后生化参数及抗体反应的变化，尚无确切信息。本研究旨在调查结膜接种布鲁氏菌病疫苗后某些生化参数水平的变化。选用30只绵羊作为动物材料。采用单剂量针对羊种布鲁氏菌的疫苗进行接种，并在4个月内从颈静脉采血。通过血清试管凝集试验测定抗体水平。使用商用自动分析仪测定血清中的碱性磷酸酶（ALP）、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、乳酸脱氢酶（LDH）、葡萄糖、总蛋白和白蛋白水平。与接种前相比，抗体滴度在第一个月显著升高（p < 0.001），但在第二个月开始下降。接种疫苗后，葡萄糖、AST、ALT活性无统计学显著变化（p > 0.005）。接种疫苗后，总蛋白和ALP的含量显著降低（p < 0.005）。LDH水平和总蛋白水平显著升高（p < 0.005）。总之，结膜疫苗被认为可安全用于保护绵羊免受布鲁氏菌病感染，本研究结果可用于提高家畜管理中布鲁氏菌病根除计划的效率。
