Hilfenhaus J, Herrmann A, Mauler R, Prince A M
Vox Sang. 1986;50(4):208-11. doi: 10.1111/j.1423-0410.1986.tb04882.x.
Heat treatment at 60 degrees C for 10 h in solution (pasteurization) was introduced into the manufacturing process of antihemophilic cryoprecipitate (AHC) and factor VIII concentrates (F VIII) to reduce the risk of transmission of hepatitis to hemophiliacs. Since the acquired immunodeficiency syndrome (AIDS) may also be transmitted to hemophiliacs by antihemophilic plasma protein preparations, we have investigated inactivation of the AIDS virus HTLV III by pasteurization in AHC or F VIII and included in this study cytomegalovirus (CMV), Epstein-Barr virus (EBV), herpes simplex virus (HSV), poliovirus and vaccinia virus. Each of these viruses was efficiently inactivated by pasteurization although considerable differences were observed between the different viruses HTLV III was rapidly inactivated, becoming nondetectable within 30-60 min. Our findings indicate that pasteurized AHC or F VIII should have a high margin of safety regarding the transmission of AIDS or any other infectious disease caused by viruses such as those tested.
在抗血友病冷沉淀（AHC）和凝血因子VIII浓缩物（F VIII）的生产过程中引入60摄氏度溶液中10小时的热处理（巴氏消毒法），以降低肝炎病毒传播给血友病患者的风险。由于获得性免疫缺陷综合征（艾滋病）也可能通过抗血友病血浆蛋白制剂传播给血友病患者，我们研究了通过巴氏消毒法在AHC或F VIII中使艾滋病病毒HTLV III失活的情况，本研究还包括巨细胞病毒（CMV）、EB病毒（EBV）、单纯疱疹病毒（HSV）、脊髓灰质炎病毒和牛痘病毒。尽管不同病毒之间存在显著差异，但每种病毒都能通过巴氏消毒法有效失活。HTLV III迅速失活，在30 - 60分钟内就检测不到了。我们的研究结果表明，经过巴氏消毒的AHC或F VIII在艾滋病或任何其他由所检测的这类病毒引起的传染病传播方面应具有很高的安全系数。
