Tu Jiawei, Gan Ying, Liang Tao, Hu Qiongwen, Wang Qian, Ren Tianling, Sun Qiyong, Wan Hao, Wang Ping
Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, China.
Tsinghua National Laboratory for Information Science and Technology, Tsinghua University, Beijing, China.
Front Chem. 2018 Aug 14;6:333. doi: 10.3389/fchem.2018.00333. eCollection 2018.
Invisible mercury ion is an extremely poisonous environmental pollutant, therefore, a fast and highly sensitive detection method is of significant importance. In this study, a liquid-gated graphene field-effect transistor (GFET) array biosensor (6 × 6 GFETs on the chip) was fabricated and applied for Hg quantitate detection based on single-stranded DNA (ssDNA) aptamer. The biosensor showed outstanding selectivity to Hg in mixed solutions containing various metal ions. Moreover, the sensing capability of the biosensor was demonstrated by real-time responses and showed a fairly low detection limit of 40 pM, a wide detection ranged from 100 pM to 100 nM and rapid response time below one second. These results suggest that the GFET array biosensor based on ssDNA aptamer offers a simple fabrication procedure and quite fast method for mercury ion contaminant detection and are promising for various analytical applications.
汞离子是一种剧毒的环境污染物，因此，快速且高灵敏度的检测方法至关重要。在本研究中，制备了一种液栅石墨烯场效应晶体管（GFET）阵列生物传感器（芯片上有6×6个GFET），并将其应用于基于单链DNA（ssDNA）适配体的汞定量检测。该生物传感器对含有各种金属离子的混合溶液中的汞表现出出色的选择性。此外，通过实时响应证明了该生物传感器的传感能力，其检测限相当低，为40 pM，检测范围宽，从100 pM到100 nM，响应时间快速，低于一秒。这些结果表明，基于ssDNA适配体的GFET阵列生物传感器提供了一种简单的制造程序和相当快速的汞离子污染物检测方法，在各种分析应用中具有广阔前景。
