Instituto de Investigación en Ciencias de la Alimentación (CIAL) (CSIC-UAM), Campus de la Universidad Autónoma de Madrid, Nicolás Cabrera 9, 28049-Madrid, Spain.
Food Funct. 2018 Sep 19;9(9):4888-4896. doi: 10.1039/c8fo01060a.
This work addresses the role of different by-products derived from the industrial extraction of orange juice in a possible anti-inflammatory effect in mice with colitis induced by dextran sulfate sodium (DSS). Fresh orange residue (FOR), dry orange residue (DOR), orange liqueur (OL) and animal feed (AF), as well as commercial citrus pectin (CP), were administered to C57BL/6J mice for 15 days before starting the DSS treatment. Analysis of macroscopic parameters such as the Disease Activity Index (DAI) and the colonic weight/length ratio revealed an anti-inflammatory effect following intake of FOR, AF or CP. Moreover, q-PCR of RNA from colonic tissue indicated measurable changes in the expression of TNF-α, IL-1β, iNOS, and intercellular adhesion molecules ICAM I, as well as in intestinal barrier proteins such as MUC-3, occludin, and ZO-1. Pectin, phenolic compounds and/or Maillard reaction products formed at initial steps were identified as relevant components exerting the ascribed beneficial effects. Our findings could open up the further application of a variety of orange by-products as food supplements in the potential amelioration of inflammatory bowel diseases.
本研究探讨了从工业提取橙汁过程中产生的不同副产物在葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠中可能的抗炎作用中的作用。新鲜橙渣（FOR）、干橙渣（DOR）、橙利口酒（OL）和动物饲料（AF）以及商业柑橘果胶（CP）在开始 DSS 处理前给 C57BL/6J 小鼠喂食 15 天。分析疾病活动指数（DAI）和结肠重量/长度比等宏观参数表明，摄入 FOR、AF 或 CP 具有抗炎作用。此外，来自结肠组织的 RNA 的 q-PCR 表明 TNF-α、IL-1β、iNOS 和细胞间黏附分子 ICAM I 的表达以及肠屏障蛋白如 MUC-3、occludin 和 ZO-1 发生了可测量的变化。在初始步骤形成的果胶、酚类化合物和/或美拉德反应产物被鉴定为发挥所赋予的有益作用的相关成分。我们的研究结果可能为各种橙副产物作为食品补充剂在炎症性肠病的潜在改善中的进一步应用开辟了道路。
