Department of Laboratory Medicine, University of Medicine and Pharmacy of Tîrgu Mureş, Gheorghe Marinescu Street, No. 38, 540136, Tîrgu Mureş, Romania.
Central Laboratory, Emergency Clinical County Hospital Tîrgu Mureş, Romania, Gheorghe Marinescu Street, No. 50, 540136, Tîrgu Mureş, Romania.
Mycopathologia. 2018 Dec;183(6):967-972. doi: 10.1007/s11046-018-0293-2. Epub 2018 Aug 30.
The present study aimed to evaluate the incidence of candidemia, the trend of species distribution and the antifungal susceptibility patterns of all invasive strains of Candida spp. isolated over a 6-year period in an Eastern European University hospital. A total number of 156 isolates were reidentified by MALDI-TOF and tested for susceptibility by Sensititre YeastOne™. Isolates were assigned as non-susceptible or belonging to the wild type according to the new CLSI (2017, 2018) break points and epidemiological cut-off values. C. parapsilosis (37.82%) was the most frequently isolated yeast, followed by C. albicans (26.28%). The general tendency of the species distribution during the surveyed period shifted towards an increase in C. parapsilosis and C. lusitaniae isolates. Fluconazole resistance was present in 23.52% of C. glabrata, 5.08% of C. parapsilosis and in none of the C. albicans, C. tropicalis and C. lusitaniae isolates. Echinocandin resistance was present only in 1(5.88%) C. glabrata isolate. The study emphasises the importance of monitoring local epidemiologic data and antifungal susceptibility trends due to the diversity of affected patient groups in our hospital.
本研究旨在评估 6 年间东欧某大学附属医院分离的所有侵袭性念珠菌属菌株的念珠菌血症发生率、种属分布趋势和抗真菌药敏模式。通过 MALDI-TOF 重新鉴定了 156 株分离株，并通过 Sensititre YeastOne™ 进行药敏测试。根据新的 CLSI（2017、2018）折点和流行病学临界点，将分离株判定为非敏感性或属于野生型。最常分离到的酵母为近平滑念珠菌（37.82%），其次为白念珠菌（26.28%）。在调查期间，种属分布的总体趋势向 C. parapsilosis 和 C. lusitaniae 分离株的增加倾斜。氟康唑耐药性存在于 23.52%的 C. glabrata、5.08%的 C. parapsilosis 中，而在白念珠菌、热带念珠菌和 C. lusitaniae 中均不存在。只有 1（5.88%）株 C. glabrata 对棘白菌素耐药。该研究强调了由于我院受影响患者群体的多样性，监测当地流行病学数据和抗真菌药敏趋势的重要性。
