Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia.
Am J Clin Pathol. 2019 Jan 1;151(1):63-67. doi: 10.1093/ajcp/aqy086.
Traditionally, for mycobacterial culture both solid and broth media are used and routinely held for 6 weeks minimum to optimize yield. We retrospectively reviewed all positive mycobacterial cultures over a 12-month period to assess growth kinetics of clinically relevant isolates.
From January to December 2015, 658 positive mycobacteria cultures by solid (7H11 and 7H10 plates) and/or broth (BACTEC MGIT) media were identified and reviewed.
In broth-only cultures, 21 of 153 (13.7%) from 21 patients were positive after 28 days' incubation. Subsequent chart review revealed the following species: 11 Mycobacterium avium intracellulare complex (MAI), five Mycobacterium tuberculosis (MTB), and five other non-MTB/MAI mycobacteria. Two of the cases of MTB were first-time isolates, and 11.4% of MTB-positive cultures became positive after 4 weeks' incubation.
These data provide strong evidence reaffirming that clinically meaningful results are frequently detected after extended incubation times by broth-only methods, including several MTB isolates.
传统上，分枝杆菌培养既使用固体培养基又使用液体培养基，并且为了优化产量通常至少孵育 6 周。我们回顾性分析了 12 个月期间所有分枝杆菌阳性培养物，以评估临床相关分离株的生长动力学。
2015 年 1 月至 12 月，通过固体（7H11 和 7H10 平板）和/或液体（BACTEC MGIT）培养基鉴定并回顾了 658 例分枝杆菌阳性培养物。
在仅液体培养物中，21 例来自 21 例患者的培养物在孵育 28 天后呈阳性。随后的图表回顾显示以下物种：11 株细胞内分枝杆菌复合体（MAC）、5 株结核分枝杆菌（MTB）和 5 株其他非 MTB/MAC 分枝杆菌。2 例 MTB 为初次分离株，并且 11.4%的 MTB 阳性培养物在孵育 4 周后呈阳性。
这些数据提供了强有力的证据，再次证实仅通过液体方法延长孵育时间后，经常可检测到有临床意义的结果，包括几种 MTB 分离株。
