针对冠状病毒229E感染细胞的抗体依赖性细胞毒性。
Antibody dependent cellular cytotoxicity against coronavirus 229E-infected cells.
作者信息
Holmes M J, Callow K A, Childs R A, Tyrrell D A
出版信息
Br J Exp Pathol. 1986 Aug;67(4):581-6.
Abstract
An antibody dependent cellular cytotoxic (ADCC) reaction was elicited using human mixed lymphocyte cultures against coronavirus 229E-infected C-16 cells. The response was assayed in microtitre plates by radioactive chromium release. Optimal killing of target cells was achieved using cells labelled 22 h after infection. A prozone was present and the optimal dilution of sera was invariably 1:200. The majority (70%) of sera tested gave a positive ADCC response. These included all sera judged positive by ELISA, but also some which were judged negative. Two experiments using purified IgG from serum which was ADCC and ELISA positive suggested that the response is mediated by an IgG antibody and that the prozone is due to a separate serum component.
摘要
利用人混合淋巴细胞培养物对冠状病毒229E感染的C - 16细胞引发抗体依赖性细胞毒性（ADCC）反应。通过放射性铬释放法在微量滴定板中检测该反应。使用感染后22小时标记的细胞实现了对靶细胞的最佳杀伤。存在前带现象，血清的最佳稀释度始终为1:200。所检测的大多数（70%）血清给出了阳性ADCC反应。这些血清包括所有经ELISA判定为阳性的血清，但也包括一些被判定为阴性的血清。两项使用来自ADCC和ELISA均呈阳性的血清纯化IgG的实验表明，该反应由IgG抗体介导，且前带现象是由一种单独的血清成分所致。
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