Research Laboratories for Health Science and Food Technologies, Kirin Company Ltd, Yokohama-shi, Kanagawa, Japan.
Research Laboratories for Health Science and Food Technologies, Kirin Company Ltd, Yokohama-shi, Kanagawa, Japan.
Neurobiol Aging. 2018 Dec;72:23-31. doi: 10.1016/j.neurobiolaging.2018.07.016. Epub 2018 Aug 6.
Alongside the rapid growth in aging populations, prevention of age-related memory decline and dementia has become a high priority. Several epidemiological and clinical studies have concluded that fermented dairy products can help to prevent cognitive decline; furthermore, intake of Camembert cheese prevents Alzheimer's pathology in model mice. To elucidate molecular mechanisms underlying the preventive effects of fermented dairy products, here we screened peptides from digested fermented dairy products for ability to improve memory function in a scopolamine-induced amnesia mouse model. We found that Trp-Tyr (WY)-containing peptides from whey protein improved memory function in the mice, and the effects were confirmed in aged mice. The WY-containing peptides directly inhibited monoamine oxidase-B activity and increased dopamine levels in brain tissue. Pretreatment with dopamine receptor antagonist abolished the improvement in memory function due to WY-containing peptides. These results suggest that WY-containing peptides in fermented dairy products increase monoamine levels by inhibiting monoamine oxidase-B activity, helping to prevent age-related cognitive decline.
随着人口老龄化的快速增长，预防与年龄相关的记忆衰退和痴呆症已成为当务之急。几项流行病学和临床研究的结论表明，发酵乳制品有助于预防认知能力下降；此外，食用卡门贝尔奶酪可预防模型小鼠的阿尔茨海默病病理。为了阐明发酵乳制品预防作用的分子机制，我们在此筛选了来自消化的发酵乳制品的肽，以研究其在东莨菪碱诱导的健忘症小鼠模型中改善记忆功能的能力。我们发现，乳清蛋白中的 Trp-Tyr（WY）肽可改善小鼠的记忆功能，并且在老年小鼠中也得到了证实。WY 肽可直接抑制单胺氧化酶-B 活性并增加脑组织中的多巴胺水平。预先用多巴胺受体拮抗剂处理可消除 WY 肽引起的记忆功能改善。这些结果表明，发酵乳制品中的 WY 肽通过抑制单胺氧化酶-B 活性增加单胺水平，有助于预防与年龄相关的认知能力下降。
