Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China.
Analyst. 2019 Jan 28;144(3):808-823. doi: 10.1039/c8an01079j.
Advances in microfluidic techniques have prompted researchers to study the inherent heterogeneity of single cells in cell populations. This would be helpful in the identification of major diseases and the design of personalized medicine. Different microfluidic approaches provide a variety of functions in the process of single-cell analysis. In this review, we take a broad overview of various microfluidic-based approaches for single-cell isolation, single-cell lysis, and single-cell analysis. Up-to-date flagship techniques and the pros and cons of these methods are discussed in detail.
微流控技术的进步促使研究人员研究细胞群体中单细胞的固有异质性。这有助于鉴定主要疾病并设计个性化药物。不同的微流控方法在单细胞分析过程中提供了多种功能。在这篇综述中，我们广泛概述了用于单细胞分离、单细胞裂解和单细胞分析的各种基于微流控的方法。详细讨论了最新的旗舰技术以及这些方法的优缺点。
