NESMOS Department - Dermatology Unit of S. Andrea Hospital, Sapienza University of Rome, Italy.
Department of Environmental Biology, Sapienza University of Rome, Italy.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Jan 5;206:547-551. doi: 10.1016/j.saa.2018.08.037. Epub 2018 Aug 21.
Nowadays, practice of tattooing is very common worldwide and, along with this increasing trend, there is also an increased risk of adverse reactions to tattoo pigments that are well known and described in literature. Previous studies have reported that cutaneous and allergic reactions to a particular pigment can manifest in several ways (allergic contact dermatitis and photo-allergic dermatitis). In this paper, a new high-throughput method is presented, in order to achieve a new system for the quality control on tattoo inks based on chromatographic-spectroscopic approach. The samples, twenty-one tattoo inks and three permanent makeup, comprised the following colors: black inks, yellow, blue, green, white, pink and various shades of red (pigment that gives many allergic responses) were analyzed through the combination of chromatographic and spectroscopic techniques, the HPTLC-Raman. In particular, Raman technique has been chosen because of its high sensitivity towards the inorganic and organic pigments, main constituents of tattoo inks. Moreover, the advantage of this hyphenated technique is to overcome the problem of analysing the complex mixture of tattoo inks, allowing to obtain a Raman spectrum of each single component, isolated by chromatographic separation. This approach aims at developing a powerful instrument to establish the nature of tattoo inks and substances that could be cause adverse reactions in tattooed patients.
如今，纹身的做法在全球范围内非常普遍，随着这种趋势的增加，纹身颜料的不良反应风险也在增加，这些不良反应在文献中已有记载和描述。以前的研究报告表明，对特定颜料的皮肤和过敏反应有多种表现形式（过敏性接触性皮炎和光变应性皮炎）。本文提出了一种新的高通量方法，旨在基于色谱-光谱方法为纹身油墨的质量控制建立一个新系统。样品包括 21 种纹身油墨和 3 种永久性彩妆，颜色有黑色、黄色、蓝色、绿色、白色、粉红色和各种深浅的红色（许多过敏反应的颜料）。通过色谱和光谱技术（HPTLC-Raman）组合分析这些样品。之所以选择 Raman 技术，是因为它对纹身油墨的主要成分——无机和有机颜料具有很高的灵敏度。此外，这种联用技术的优势在于能够克服分析纹身油墨复杂混合物的问题，允许通过色谱分离获得每个单一成分的 Raman 光谱。这种方法旨在开发一种强大的仪器，以确定纹身油墨的性质以及可能导致纹身患者不良反应的物质。
