Freyssinet J M, Wiesel M L, Gauchy J, Boneu B, Cazenave J P
Thromb Haemost. 1986 Jun 30;55(3):309-13.
An anticoagulant activity was isolated from the plasma of a patient with a strong lupus-like anticoagulant using gel filtration by high performance liquid chromatography. IgM were detected in this anticoagulant fraction which exhibited specificity towards 50% phosphatidylcholine - 50% phosphatidylserine vesicles and cardiolipin. These phospholipids were able to produce an apparent 3-fold enhancement of purified human protein C activation by human alpha-thrombin in the presence of purified human placenta thrombomodulin. In the absence of phospholipid, the anticoagulant fraction had no effect on thrombomodulin activity. The anticoagulant fraction could neutralize the enhancement of thrombomodulin activity by phospholipid in a dose-dependent manner. This study suggests that the neutralization of phospholipid might result in a reduced activation of protein C which could be responsible for the occurrence of thrombotic complications in a proportion of patients with lupus anticoagulants.
使用高效液相色谱凝胶过滤法，从一名患有强狼疮抗凝物的患者血浆中分离出一种抗凝活性物质。在该抗凝物组分中检测到IgM，其对50%磷脂酰胆碱 - 50%磷脂酰丝氨酸囊泡和心磷脂具有特异性。在纯化的人胎盘血栓调节蛋白存在的情况下，这些磷脂能够使人类α-凝血酶对纯化的人蛋白C的激活作用明显增强3倍。在没有磷脂的情况下，抗凝物组分对血栓调节蛋白活性没有影响。抗凝物组分能够以剂量依赖的方式中和磷脂对血栓调节蛋白活性的增强作用。这项研究表明，磷脂的中和可能导致蛋白C激活减少，这可能是一部分狼疮抗凝物患者发生血栓并发症的原因。
