Johnson C A, Snider T G, Henk W G, Fulton R W
Vet Pathol. 1986 Jul;23(4):443-53. doi: 10.1177/030098588602300415.
Neonatal gnotobiotic dogs orally inoculated with canine rotavirus had ultrastructural changes limited to the jejunal and ileal regions of the small intestine. Early scanning electron microscopic findings consisted of swollen villus epithelial cells, denuded foci on intestinal villi, and slight to moderate villus atrophy. Later changes were slight villus atrophy with no denuded intestinal villi. Transmission electron microscopic changes in villus epithelial cells from 12 to 48 hours post-inoculation included: rotavirus particles associated with intracytoplasmic vacuoles near the terminal web and apical tubules; viral particles in dilated cisternae of rough endoplasmic reticulum; and moderate numbers of necrotic cells having no microvilli, swollen mitochondria, membrane-bound lipid-like material in the cytoplasm, clumped chromatin around the periphery of the nucleus, and disruption of the cytoplasmic membrane. In jejunum and ileum at 72 to 154 hours post-inoculation, there were fewer necrotic villus epithelial cells and fewer virus particles. In addition, the ultrastructural morphology of the majority of the villus epithelial cells was similar to crypt epithelium. These studies showed that rotavirus infected the villus epithelial cells with subsequent propagation of the rotavirus and destruction of villus epithelial cells.
经口接种犬轮状病毒的新生无菌犬，其超微结构变化仅限于小肠的空肠和回肠区域。早期扫描电子显微镜检查结果包括绒毛上皮细胞肿胀、肠绒毛上的剥脱灶以及轻度至中度的绒毛萎缩。后期变化为轻度绒毛萎缩，无肠绒毛剥脱。接种后12至48小时，绒毛上皮细胞的透射电子显微镜变化包括：轮状病毒颗粒与终末网和顶端小管附近的胞质空泡相关；病毒颗粒存在于粗面内质网的扩张池中；大量坏死细胞无微绒毛、线粒体肿胀、胞质中有膜结合的类脂物质、核周染色质聚集以及细胞膜破坏。接种后72至154小时，空肠和回肠中坏死的绒毛上皮细胞和病毒颗粒较少。此外，大多数绒毛上皮细胞的超微结构形态与隐窝上皮相似。这些研究表明，轮状病毒感染绒毛上皮细胞，随后轮状病毒繁殖并破坏绒毛上皮细胞。
