Pearson G R, McNulty M S
Arch Virol. 1979;59(1-2):127-36. doi: 10.1007/BF01317902.
The small intestine of piglets orally infected with rotavirus was examined by electron microscopy 18, 24, 48 and 60 hours after infection. At 18 and 24 hours after infection columnar epithelial cells covered the villi. Infected epithelial cells tended to be less electron-dense than uninfected cells and were more numerous at 24 hours after infection. Two types of rotavirus particle were seen, usually within dilated cisternae of the RER: non-enveloped particles measuring 50 to 60 nm and enveloped particles measuring 65 to 75 nm. Non-membrane bound viroplasm containing electron-dense cores was encountered outside the cisternae 18 and 24 hours after infection. Tubular structures measuring 44 to 56 nm were often found in nuclei of infected cells. Single-membraned (44 to 55 nm) and double-membraned tubules (70 to 80 nm) associated with viral manufacture were found in the cytoplasm of infected cells. At 48 and 60 hours after infection a proportion of villous epithelial cells were cuboidal. Virus particles were detected in only a few epithelial cells and nuclear and cytoplasmic tubules were not seen. At all times after infection some infected cells showed a reduction in the number and size of the microvilli comprising the brush border.
在感染轮状病毒的仔猪感染后18、24、48和60小时，通过电子显微镜对其小肠进行检查。感染后18和24小时，绒毛被柱状上皮细胞覆盖。受感染的上皮细胞往往比未受感染的细胞电子密度低，且在感染后24小时数量更多。可见两种类型的轮状病毒颗粒，通常位于粗面内质网扩张的潴泡内：无包膜颗粒直径为50至60纳米，有包膜颗粒直径为65至75纳米。感染后18和24小时，在潴泡外可见含有电子致密核心的无膜结合病毒质。在受感染细胞的细胞核中经常发现直径为44至56纳米的管状结构。在受感染细胞的细胞质中发现了与病毒制造相关的单膜（44至55纳米）和双膜小管（70至80纳米）。感染后48和60小时，一部分绒毛上皮细胞呈立方形。仅在少数上皮细胞中检测到病毒颗粒，未见到核小管和胞质小管。在感染后的所有时间里，一些受感染细胞的微绒毛数量和大小减少，微绒毛构成刷状缘。
