Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, China.
Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, China.
Front Immunol. 2018 Aug 24;9:1845. doi: 10.3389/fimmu.2018.01845. eCollection 2018.
Duck hepatitis A virus type 1 (DHAV-1) is one of the most common and lethal pathogens in young ducklings. Live-attenuated DHAV vaccine (CH60 strain) developed by passaging in chicken embryos provided effective immune protection for ducklings. However, the accurate mechanism for such adaption in chicken embryos is not fully revealed. Here, we utilize RNA-sequencing to perform global transcriptional analysis of DHAV-1-innoculated embryonated livers along with histopathological and ultrastructural analysis. This study revealed that infection with DHAV-1 strain CH60 is associated with enhanced type I and II interferon responses, activated innate immune responses, elevated levels of suppressor of cytokine signaling 1 and 3 ( and ) accompanied with abnormalities in multiple metabolic pathways. Excessive inflammatory and innate immune responses induced by the CH60 strain are related to severe liver damage. Our study presents a comprehensive characterization of the transcriptome of chicken embryos infected with DHAV-CH60 and provides insight for in-depth exploration of viral adaption and virus-host interactions.
鸭甲型肝炎病毒 1 型（DHAV-1）是雏鸭中最常见和最致命的病原体之一。通过在鸡胚中传代开发的减毒 DHAV 疫苗（CH60 株）为雏鸭提供了有效的免疫保护。然而，这种在鸡胚中的适应性的确切机制尚未完全揭示。在这里，我们利用 RNA 测序对感染 DHAV-1 株 CH60 的鸡胚进行了全局转录组分析，并结合组织病理学和超微结构分析。本研究表明，DHAV-1 株 CH60 的感染与增强的 I 型和 II 型干扰素反应、激活的先天免疫反应、细胞因子信号转导抑制因子 1 和 3（和）水平升高以及多种代谢途径异常有关。CH60 株引起的过度炎症和先天免疫反应与严重的肝损伤有关。本研究全面描述了感染 DHAV-CH60 的鸡胚的转录组，为深入探索病毒适应性和病毒-宿主相互作用提供了依据。
