MiMedx Group, Inc., Marietta, Georgia 30062.
J Biomed Mater Res B Appl Biomater. 2019 May;107(4):1035-1046. doi: 10.1002/jbm.b.34196. Epub 2018 Sep 10.
Chronic wounds are a significant health care problem with serious implications for quality of life because they do not properly heal and often require therapeutic intervention. Amniotic membrane allografts have been successfully used as a biologic therapy to promote soft tissue healing; however, the umbilical cord, another placental-derived tissue, has also recently garnered interest because of its unique composition but similar placental tissue origin. The aim of this study was to characterize PURION® PLUS Processed dehydrated human umbilical cord (dHUC) and evaluate the biological properties of this tissue that contribute to healing. This was performed through the characterization of the tissue composition, evaluation of in vitro cellular response to dHUC treatment, and in vivo bioresorption and tissue response in a rat model. It was observed that dHUC contains collagen I, hyaluronic acid, laminin, and fibronectin. Additionally, 461 proteins that consist of growth factors and cytokines, inflammatory modulators, chemokines, proteases and inhibitors, adhesion molecules, signaling receptors, membrane-bound proteins, and other soluble regulators were detected. Cell-based assays demonstrated an increase in adipose-derived stem cell and mesenchymal stem cell proliferation, fibroblast migration and endothelial progenitor cell vessel formation in a dose-dependent manner after dHUC treatment. Lastly, rat subcutaneous implantation demonstrated biocompatibility since dHUC allografts were resorbed without fibrous encapsulation. These findings establish that dHUC possesses biological properties that stimulate cellular responses important for soft tissue healing. © 2018 The Authors. Journal Of Biomedical Materials Research Part B: Applied Biomaterials Published By Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1035-1046, 2019.
慢性伤口是一个严重的医疗保健问题，对生活质量有严重影响，因为它们不能正常愈合，经常需要治疗干预。羊膜同种异体移植物已成功用作生物治疗来促进软组织愈合；然而，脐带，另一种胎盘来源的组织，也因其独特的组成但类似的胎盘组织起源而最近引起了关注。本研究的目的是表征 PURION® PLUS 处理的脱水人脐带 (dHUC)，并评估促进愈合的组织生物学特性。这是通过组织成分的表征、对 dHUC 处理的体外细胞反应的评估以及在大鼠模型中的体内生物吸收和组织反应来完成的。观察到 dHUC 含有胶原 I、透明质酸、层粘连蛋白和纤维连接蛋白。此外，还检测到 461 种蛋白质，这些蛋白质包括生长因子和细胞因子、炎症调节剂、趋化因子、蛋白酶和抑制剂、粘附分子、信号转导受体、膜结合蛋白和其他可溶性调节剂。基于细胞的测定表明，在脂肪源性干细胞和间充质干细胞增殖、成纤维细胞迁移和内皮祖细胞血管形成方面，dHUC 处理后呈剂量依赖性增加。最后，大鼠皮下植入显示出生物相容性，因为 dHUC 同种异体移植物在没有纤维包裹的情况下被吸收。这些发现表明 dHUC 具有刺激对软组织愈合很重要的细胞反应的生物学特性。©2018 作者。生物医学材料研究杂志 B 部分：应用生物材料由 Wiley 期刊出版，Inc. J Biomed Mater Res Part B: Appl Biomater 107B:1035-1046，2019 年。
