State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, China.
School of Biology and Blialogical Engineering, South China University of Technology, Guangzhou, 510006, China.
Curr Microbiol. 2024 Aug 1;81(9):291. doi: 10.1007/s00284-024-03816-4.
A novel Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-motile, and rod-shaped bacterium with ibuprofen-degrading capacity, designated DM4, was isolated from the sewage of a wastewater treatment plant (WWTP) in Guangzhou city, China. Strain DM4 grew optimally at 0% (w/v) NaCl, pH 5.0-7.0, and 30 °C, forming white colonies on trypticase soy agar. Cω9c, Cω9.12c and Cω10c were the predominant fatty acids. Results of 16S rRNA gene alignment and phylogenetic analysis indicated that strain DM4 belonged to the genus Patulibacter, was closely related to Patulibacter medicamentivorans DSM 25692 (98.5%) and P. brassicae KCTC 39817 (98.1%). Strain DM4 had a genome size of 5.33Mbp, and the DNA G + C content was 75.0%. The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridisation (dDDH) values between strain DM4 and P. medicamentivorans were 85.2%, 83.9%, and 29.0% respectively, while those between strain DM4 and P. brassicae were 78.5%, 71.3%, and 22.2%, respectively. Strain DM4 could significantly degrade ibuprofen by almost 80% after 84 h of incubation, and the degradation kinetics was well fitted with the first-order kinetics. Evidence from phenotypic, phylogenetic and chemotaxonomic analyses support that strain DM4 (= GDMCC 1.4574 = KCTC 59145) represents a new species of the genus Patulibacter, for which the name Patulibacter defluvii sp. nov. is proposed.
一株具有布洛芬降解能力的新型革兰氏阳性、需氧、过氧化氢酶阳性、氧化酶阴性、非运动、杆状细菌，被命名为 DM4，从中国广州市一家废水处理厂（WWTP）的污水中分离得到。菌株 DM4 在 0%（w/v）NaCl、pH5.0-7.0 和 30°C 下生长最佳，在胰蛋白酶大豆琼脂上形成白色菌落。Cω9c、Cω9.12c 和 Cω10c 是主要的脂肪酸。16S rRNA 基因序列比对和系统发育分析结果表明，菌株 DM4 属于 Patulibacter 属，与 Patulibacter medicamentivorans DSM 25692（98.5%）和 P. brassicae KCTC 39817（98.1%）密切相关。菌株 DM4 的基因组大小为 5.33Mbp，DNA G+C 含量为 75.0%。菌株 DM4 与 P. medicamentivorans 的平均核苷酸同一性（ANI）、平均氨基酸同一性（AAI）和数字 DNA-DNA 杂交（dDDH）值分别为 85.2%、83.9%和 29.0%，而与 P. brassicae 的分别为 78.5%、71.3%和 22.2%。菌株 DM4 在 84 h 的孵育后可以显著降解近 80%的布洛芬，降解动力学很好地符合一级动力学。表型、系统发育和化学分类学分析的证据支持菌株 DM4（=GDMCC 1.4574=KCTC 59145）代表 Patulibacter 属的一个新种，现命名为 Patulibacter defluvii sp. nov.。
