Kawashima M, Favre M, Jablonska S, Obalek S, Orth G
Virology. 1986 Oct 30;154(2):389-94. doi: 10.1016/0042-6822(86)90464-2.
Human papillomavirus (HPV) DNA sequences, related to the genomes of HPVs associated with epidermodysplasia verruciformis (EV), were detected in DNA samples extracted from biopsied lesions in 2 of 24 cases of actinic keratosis found in the general population. An HPV DNA was molecularly cloned from one of these samples. Blot hybridization experiments, performed under stringent conditions, revealed a significant cross-hybridization only between this HPV DNA and the DNAs of HPV5 and of the HPV5-related types. The extent of homology between them ranged from 7 to 30%, as evaluated by hybridization in liquid phase at saturation followed by nuclease S1 analysis. This showed that the cloned HPV represented a new type, tentatively named HPV36. HPV36 was not found in the other 22 cases of actinic keratosis, but was detected in scrapings of benign lesions of 7 of 18 (39%) EV patients.
在从普通人群中发现的24例光化性角化病的活检病变中提取的DNA样本中，检测到了与疣状表皮发育不良（EV）相关的人乳头瘤病毒（HPV）DNA序列。从其中一个样本中对一种HPV DNA进行了分子克隆。在严格条件下进行的印迹杂交实验显示，只有这种HPV DNA与HPV5及HPV5相关类型的DNA之间存在显著的交叉杂交。通过饱和液相杂交后进行核酸酶S1分析评估，它们之间的同源程度在7%至30%之间。这表明克隆的HPV代表一种新型，暂命名为HPV36。在其他22例光化性角化病中未发现HPV36，但在18例（39%）EV患者的7例良性病变刮片中检测到了HPV36。
