Duffy L C, Riepenhoff-Talty M, Byers T E, La Scolea L J, Zielezny M A, Dryja D M, Ogra P L
Am J Dis Child. 1986 Nov;140(11):1164-8. doi: 10.1001/archpedi.1986.02140250090041.
A cohort of 197 infants was followed up prospectively for a single rotavirus (RV) season, 1983 to 1984, to examine the effect of long-term feeding method on RV infection. The feeding classification distinguished breast vs formula milk intake over the long term, for at least 18 weeks from birth (approximately four months). During the follow-up period, relative numbers of RV particles in feces were compared by electron microscopy, and positive specimens were confirmed by an enzyme-linked immunosorbent assay. There was no apparent difference in the infection rates of rotavirus enteritis in breast-fed (20%) as compared with bottle-fed (17%) infants. However, clinical manifestation of illness was milder in breast-fed infants. Among the breast-fed subjects, fecal flora identified by bacterial cultures, biochemical reaction, and gas-liquid chromatography revealed a significant growth of bifidobacteria lasting as long as the period of lactation. Colonization by this organism above the detection level of log 10(5)/mL was not observed in the feces of bottle-fed infants. These data suggest that alterations in enteric flora induced by breast-feeding may be correlates of intraluminal events, mediated by human milk, that modulate the clinical course of RV gastroenteritis.
对197名婴儿进行了前瞻性随访，观察了1983年至1984年单个轮状病毒（RV）流行季节长期喂养方式对RV感染的影响。喂养分类区分了从出生起至少18周（约四个月）的长期母乳喂养与配方奶喂养情况。在随访期间，通过电子显微镜比较粪便中RV颗粒的相对数量，并通过酶联免疫吸附测定法确认阳性标本。母乳喂养婴儿（20%）与奶瓶喂养婴儿（17%）的轮状病毒肠炎感染率没有明显差异。然而，母乳喂养婴儿的疾病临床表现较轻。在母乳喂养的受试者中，通过细菌培养、生化反应和气液色谱法鉴定的粪便菌群显示，双歧杆菌显著生长，持续时间与哺乳期相同。在奶瓶喂养婴儿的粪便中未观察到该菌在检测水平log 10(5)/mL以上的定植。这些数据表明，母乳喂养引起的肠道菌群改变可能与母乳介导的管腔内事件相关，这些事件调节了RV胃肠炎的临床病程。
