Marbacher Serge, Grüter Basil, Schöpf Salome, Croci Davide, Nevzati Edin, D'Alonzo Donato, Lattmann Jacqueline, Roth Tabitha, Bircher Benjamin, Wolfert Christina, Muroi Carl, Dutilh Gilles, Widmer Hans Rudolf, Fandino Javier
Department of Neurosurgery c/o Neuro Research Office, Kantonsspital Aarau, Tellstrasse 1, 5001, Aarau, Switzerland.
Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, Bern, Switzerland.
Transl Stroke Res. 2018 Sep 12. doi: 10.1007/s12975-018-0657-4.
In preclinical models, modification of experimental parameters associated with techniques of inducing subarachnoid hemorrhage (SAH) can greatly affect outcomes. To analyze how parameter choice affects the relevance and comparability of findings, we systematically reviewed 765 experimental studies of in vivo animal SAH models (2000-2014). During the last decade, we found marked increases in publications using smaller species and models for simulating acute events after SAH. Overall, the fewer types of species and models used did not correlate with an increased standardization in the experimental characteristics and procedures. However, by species, commonly applied, reliable parameters for each experimental SAH technique were identified in mouse, rat, rabbit, and dog models. Our findings can serve as a starting point for discussion toward a more uniform performance of SAH experiments, development of preclinical SAH common data elements, and establishment of standardized protocols for multicenter preclinical trials.
在临床前模型中，与诱导蛛网膜下腔出血（SAH）技术相关的实验参数的改变会极大地影响实验结果。为了分析参数选择如何影响研究结果的相关性和可比性，我们系统回顾了765项体内动物SAH模型的实验研究（2000 - 2014年）。在过去十年中，我们发现使用较小物种和模拟SAH后急性事件的模型的出版物显著增加。总体而言，使用的物种和模型类型越少，与实验特征和程序标准化程度的提高并无关联。然而，按物种分类，在小鼠、大鼠、兔子和狗模型中确定了每种实验性SAH技术常用的可靠参数。我们的研究结果可作为一个起点，用于讨论如何更统一地开展SAH实验、开发临床前SAH通用数据元素以及建立多中心临床前试验的标准化方案。
