Dai Hongwei, Zhou Jiao, Zhu Bo
Department of Blood Transfusion, Suizhou Hospital, Hubei University of Medicine, Hubei, China.
Medicine (Baltimore). 2018 Sep;97(37):e12018. doi: 10.1097/MD.0000000000012018.
End-stage renal disease (ESRD) is the final stage of chronic kidney disease in which the kidney is not sufficient to meet the needs of daily life. It is necessary to understand the role of genes expression involved in ESRD patient responses to nocturnal hemodialysis (NHD) and to improve the immunity responsiveness. The aim of this study was to investigate novel immune-associated genes that may play important roles in patients with ESRD.The microarray expression profiles of peripheral blood in patients with ESRD before and after NHD were analyzed by network-based approaches, and then using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway analysis to explore the biological process and molecular functions of differentially expressed genes. Subsequently, a transcriptional regulatory network of the core genes and the connected transcriptional regulators was constructed. We found that NHD had a significant effect on neutrophil activation and immune response in patients with ESRD.In addition, Our findings suggest that MAPKAPK3, RHOA, ARRB2, FLOT1, MYH9, PRKCD, RHOG, PTPN6, MAPK3, CNPY3, PI3KCG, and PYGL genes maybe potential targets regulated by core transcriptional factors, including ARNT, C/EBPalpha, CEBPA, CREB1, PSG1, DAND5, SP1, GATA1, MYC, EGR2, and EGR3.
终末期肾病（ESRD）是慢性肾病的最后阶段，此时肾脏已无法满足日常生活的需求。了解参与ESRD患者对夜间血液透析（NHD）反应的基因表达作用并提高免疫反应性很有必要。本研究的目的是调查可能在ESRD患者中发挥重要作用的新型免疫相关基因。通过基于网络的方法分析了ESRD患者在NHD前后外周血的微阵列表达谱，然后使用基因本体论（GO）和京都基因与基因组百科全书通路分析来探索差异表达基因的生物学过程和分子功能。随后，构建了核心基因与相连转录调节因子的转录调控网络。我们发现NHD对ESRD患者的中性粒细胞活化和免疫反应有显著影响。此外，我们的研究结果表明，MAPKAPK3、RHOA、ARRB2、FLOT1、MYH9、PRKCD、RHOG、PTPN6、MAPK3、CNPY3、PI3KCG和PYGL基因可能是包括ARNT、C/EBPα、CEBPA、CREB1、PSG1、DAND5、SP1、GATA1、MYC、EGR2和EGR3在内的核心转录因子调控的潜在靶点。
