White L A, Freeman C Y, Forrester B D, Chappell W A
J Clin Microbiol. 1986 Oct;24(4):527-31. doi: 10.1128/jcm.24.4.527-531.1986.
Suspensions of herpes simplex virus types 1 and 2, cytomegalovirus, and parainfluenzavirus type 2 were inactivated within 24 h when treated at 37 degrees C with 1 mg (5.05 mM) of copper-catalyzed sodium ascorbate per ml. The infectivity titer of respiratory syncytial virus was reduced substantially after 24 h but required 48 h for inactivation. Under these conditions, inactivation of these viruses was also successfully achieved with 5.68 mM catalyzed ascorbic acid. Copper (Cu2+), when added with the ascorbate solution at 5 micrograms/ml (0.022 mM), exhibited a catalytic effect on the inactivation of these viruses. The rate of inactivation was affected by the incubation temperature, time of exposure, and the virus concentration. Ascorbate concentrations as high as 10 mg/ml (50.5 mM) demonstrated only a minimum increase in effect on viral inactivation. The loss of infectivity did not alter either the hemagglutination or complement fixation qualities of the antigens.
1型和2型单纯疱疹病毒、巨细胞病毒以及2型副流感病毒的悬液，于37℃用每毫升1毫克（5.05毫摩尔）铜催化抗坏血酸钠处理时，在24小时内即被灭活。呼吸道合胞病毒的感染性滴度在24小时后大幅降低，但需要48小时才能被灭活。在这些条件下，用5.68毫摩尔催化抗坏血酸也成功实现了这些病毒的灭活。铜（Cu2+）以每毫升5微克（0.022毫摩尔）与抗坏血酸溶液一起添加时，对这些病毒的灭活表现出催化作用。灭活速率受孵育温度、暴露时间和病毒浓度的影响。高达10毫克/毫升（50.5毫摩尔）的抗坏血酸浓度对病毒灭活的效果仅显示出最小程度的增加。感染性的丧失并未改变抗原的血凝或补体结合特性。
