Knox G E, Reynolds D W, Cohen S, Alford C A
J Clin Invest. 1978 Jun;61(6):1635-44. doi: 10.1172/JCI109084.
Pretreatment (12-48 h) of human fibroblasts with crude, human chorionic gonadotropin (HCG) was found to suppress cytomegalovirus infection and enhance productive herpes simplex type 1 (HSV) infection in vitro. Maximal effect on virus replication occurred at the time of maximal infectivity of control cultures (48 h and 6 days after viral innoculation for HSV and cytomegalovirus, respectively). The alteration in viral growth was not due to the HCG itself, but rather to epidermal growth factor, a contaminant of crude HCG. The effect of epidermal growth factor on viral infectivity was shown to be a cell-mediated event requiring protein synthesis.
研究发现，用人绒毛膜促性腺激素（HCG）粗制品对人成纤维细胞进行预处理（12 - 48小时），可在体外抑制巨细胞病毒感染，并增强单纯疱疹病毒1型（HSV）的增殖性感染。对病毒复制的最大影响发生在对照培养物达到最大感染性时（分别在接种HSV和巨细胞病毒后48小时和6天）。病毒生长的改变并非由于HCG本身，而是由于表皮生长因子，它是HCG粗制品中的一种污染物。表皮生长因子对病毒感染性的影响被证明是一个需要蛋白质合成的细胞介导事件。
