Department of Endodontics, São Paulo State University (UNESP) School of Dentistry, Araçatuba, Brazil.
Department of Basic Sciences, São Paulo State University (UNESP) School of Dentistry, Araçatuba, Brazil.
Int Endod J. 2019 Mar;52(3):329-336. doi: 10.1111/iej.13014. Epub 2018 Sep 26.
To investigate the effect of chronic alcohol consumption on apical periodontitis in rats.
Thirty-two male Wistar rats were arranged into four groups: Control (C): without apical periodontitis and nonalcoholic diet; (AL): without apical periodontitis and alcoholic diet; (AP): with apical periodontitis and nonalcoholic diet; and (AP + AL): with apical periodontitis and alcoholic diet. The alcoholic solution at 20% was given to the AL and AP + AL groups as the sole source of hydration throughout the experiment. AP was induced in the mandibular left first molars at the end of the 4th week. Weight changes and the amount of solid and liquid foods were recorded for 8 weeks. At the end, the animals were euthanized and the jaws removed followed by histological processing for histopathological and RANKL, OPG, TRAP and HIF-1α analyses. The Mann-Whitney test was used for nonparametric data, and anova followed by the Tukey test was performed for parametric data, with P < 0.05.
Animals that received the alcoholic diet had a lower weight gain than the other groups (P < 0.05). Control and AL groups did not have an inflammatory response in the periapical tissues. The median score of inflammatory infiltrate was significantly higher in the AP + AL group (2.5) compared to the AP group (1.5; P < 0.05). In the same comparison, AP + AL was associated with score 3 for RANKL and HIF-1α versus score 2 for AP group (P < 0.05). Moreover, the values for TRAP were 3.88 ± 0.70 cells mm for the AP + AL group and 2.43 ± 0.94 cells mm for the AP group (P < 0.05).
In rats, an alcoholic diet had a significant effect on the severity of apical periodontitis, exacerbating the inflammatory response and osteoclastogenesis.
研究慢性酒精摄入对大鼠根尖周炎的影响。
将 32 只雄性 Wistar 大鼠分为四组：对照组（C）：无根尖周炎和非酒精饮食；（AL）：无根尖周炎和酒精饮食；（AP）：有根尖周炎和非酒精饮食；和（AP+AL）：有根尖周炎和酒精饮食。在整个实验过程中，AL 和 AP+AL 组仅用 20%的酒精溶液作为唯一的水分来源。在第 4 周末，在下颌左侧第一磨牙诱导根尖周炎。记录 8 周的体重变化和固体及液体食物的摄入量。最后，处死动物，取出颌骨，进行组织学处理，进行组织病理学和 RANKL、OPG、TRAP 和 HIF-1α 分析。非参数数据采用 Mann-Whitney 检验，参数数据采用方差分析加 Tukey 检验，P<0.05。
接受酒精饮食的动物体重增加低于其他组（P<0.05）。对照组和 AL 组根尖周组织无炎症反应。与 AP 组（1.5）相比，AP+AL 组炎症浸润中位数评分（2.5）显著升高（P<0.05）。在相同的比较中，AP+AL 与 RANKL 和 HIF-1α 的评分 3 相关，而 AP 组的评分 2（P<0.05）。此外，AP+AL 组的 TRAP 值为 3.88±0.70 个细胞 mm，AP 组为 2.43±0.94 个细胞 mm（P<0.05）。
在大鼠中，酒精饮食对根尖周炎的严重程度有显著影响，加重了炎症反应和破骨细胞生成。
