School of Science, State Key Laboratory for Mechanical Behavior of Materials, MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Materials, Instrumental Analysis Center, Xi'an Jiaotong University, Xi'an 710049, PR China.
School of Science, State Key Laboratory for Mechanical Behavior of Materials, MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Materials, Instrumental Analysis Center, Xi'an Jiaotong University, Xi'an 710049, PR China.
Biosens Bioelectron. 2018 Dec 15;121:159-165. doi: 10.1016/j.bios.2018.08.004. Epub 2018 Aug 4.
We describe a 3-dimensional scaffold nanocomposite oxidase nanozyme (termed PtNPs@MnO) formed from 2-dimensional MnO nanosheets grown on the surface of large platinum nanoparticles (PtNPs). The two components act synergistically in the catalytic oxidation of 3,3',5,5'-tetramethylbenzidine (TMB), due to the intimate contact between the two components and mutual electron transfer phenomena. The Wonton-like 3-dimensional structure of the nanocomposite is highly stable and was characterized by cryogenic electron microscopy (cryo-EM). The PtNPs@MnO nanocomposite was employed in a sensing assay for the determination of glutathione (GSH) and dopamine (DA) with a linear range of 0.2 μM-11 μM for GSH and 0.1 μM-1.1 μM for DA. The limit of detection (LOD) and limit of quantification (LOQ) of GSH are 0.05 μM (S/N = 3) and 0.17 μM (S/N = 10), respectively. The LOD and LOQ for DA were estimated as 0.025 μM and 0.083 μM. The method was applied to analyze GSH in human serum samples and DA in pharmaceuticals.
我们描述了一种由二维 MnO 纳米片在大尺寸铂纳米颗粒（PtNPs）表面生长而成的三维支架纳米复合材料氧化酶纳米酶（称为 PtNPs@MnO）。由于两种组分之间的紧密接触和相互电子转移现象，两种组分在 3,3',5,5'-四甲基联苯胺（TMB）的催化氧化中协同作用。该纳米复合材料的馄饨状三维结构非常稳定，并通过低温电子显微镜（cryo-EM）进行了表征。PtNPs@MnO 纳米复合材料被用于测定谷胱甘肽（GSH）和多巴胺（DA）的传感测定中，对于 GSH 的线性范围为 0.2 μM-11 μM，对于 DA 的线性范围为 0.1 μM-1.1 μM。GSH 的检测限（LOD）和定量限（LOQ）分别为 0.05 μM（S/N=3）和 0.17 μM（S/N=10）。DA 的 LOD 和 LOQ 分别估计为 0.025 μM 和 0.083 μM。该方法用于分析人血清样品中的 GSH 和药物中的 DA。
