胍在体外对脊髓灰质炎病毒聚合酶的抑制作用。
Inhibition of poliovirus polymerase by guanidine in vitro.
作者信息
Tershak D R
出版信息
J Virol. 1982 Jan;41(1):313-8. doi: 10.1128/JVI.41.1.313-318.1982.
Abstract
Extracts from poliovirus-infected HeLa cells synthesized 35s viral RNA, replicative intermediate, and double-stranded RNA in vitro. Guanidine inhibited the synthesis of all three species of RNA; production of 35s RNA was most sensitive to the inhibitor. Pulse-chase experiments with [3H]UTP indicated that guanidine had no detectable effect on elongation of polynucleotide chains or the release of completed RNA chains from the viral replication complex. Experiments in which short pulses of precursor were used suggest that guanidine blocked the initiation step of RNA synthesis in vitro.
摘要
从感染脊髓灰质炎病毒的海拉细胞中提取的物质在体外合成了35S病毒RNA、复制中间体和双链RNA。胍抑制了这三种RNA的合成；35S RNA的产生对该抑制剂最为敏感。用[3H]UTP进行的脉冲追踪实验表明，胍对多核苷酸链的延伸或完整RNA链从病毒复制复合物中的释放没有可检测到的影响。使用前体短脉冲的实验表明，胍在体外阻断了RNA合成的起始步骤。
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