胰蛋白酶对人轮状病毒生长和感染性的影响。

The effect of trypsin on the growth and infectivity of human rotavirus.

作者信息

Kitaoka S, Suzuki H, Numazaki Y, Konno T, Ishida N

出版信息

Tohoku J Exp Med. 1986 Aug;149(4):437-47. doi: 10.1620/tjem.149.437.

DOI:10.1620/tjem.149.437

Abstract

The effect of trypsin on the infectivity of human rotavirus (HRV) was examined using HRV strains that are cultivable only when treated with trypsin. Their infectivity in cell culture systems was enhanced in relation to enzyme concentration. The primary effect of trypsin appeared to be on the virus. Trypsin untreated HRV virions were capable of being adsorbed on the cell (MA104) but not of passing into it without trypsin. For multiple cycles of replication the presence of trypsin in the maintenance medium was required.

摘要

使用仅在经胰蛋白酶处理时才可培养的人轮状病毒（HRV）毒株，研究了胰蛋白酶对HRV感染性的影响。在细胞培养系统中，其感染性随酶浓度升高而增强。胰蛋白酶的主要作用似乎是作用于病毒。未经胰蛋白酶处理的HRV病毒粒子能够吸附在细胞（MA104）上，但在没有胰蛋白酶的情况下无法进入细胞。在维持培养基中需要有胰蛋白酶才能进行多轮复制。