Chang Tien-Lin, Huang Tzu-Wen, Wang Ying-Xuan, Liu Chang-Pan, Kirby Ralph, Chu Chien-Ming, Huang Chih-Hung
Graduate Institution of Engineering Technology-Doctoral Department, National Taipei University of Technology, Taipei 10608, Taiwan.
Institute of Biochemical and Biomedical Engineering, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 10608, Taiwan.
Microorganisms. 2021 Mar 18;9(3):630. doi: 10.3390/microorganisms9030630.
The need for new antibiotics is increasing due to their overuse, and antibiotic resistance has become one of the major threats worldwide to public health, food safety, and clinical treatment. In this study, we describe an actinobacterial isolate, YX44, which belongs to the genus . This was isolated from a drinking pipe located in Osaka, Japan, and has the ability to inhibit Gram-positive bacteria, Gram-negative bacteria, and various fungi. YX44 fermentation broth shows strong activity against and , as well as also inhibiting clinical isolates of multidrug-resistant . The YX44 antibacterial substances in the broth are relatively heat-stable, show high stability from the pH range 1 to 11, and have good solubility in both organic and non-organic solvents. Size-exclusion chromatography revealed that the YX44 antibacterial compounds are less than 1000 Da in size. LC-MS was able to identify three possible candidate molecules with molecular weights of 308, 365, 460, and 653 g/mol; none of these sizes correspond to any well-known antibiotics. Our results show that YX44 seems to produce a number of novel antibiotics with high pH stability and good solubility that have significant activity against including multidrug-resistant strains.
由于抗生素的过度使用，对新型抗生素的需求日益增加，而抗生素耐药性已成为全球范围内对公共卫生、食品安全和临床治疗的主要威胁之一。在本研究中，我们描述了一种放线菌分离株YX44，它属于 属。该菌株从日本大阪的一根饮用水管中分离得到，具有抑制革兰氏阳性菌、革兰氏阴性菌和多种真菌的能力。YX44发酵液对 和 显示出强大的活性，同时也能抑制多重耐药临床分离株。发酵液中的YX44抗菌物质相对耐热，在pH值1至11的范围内具有较高的稳定性，并且在有机溶剂和非有机溶剂中都具有良好的溶解性。尺寸排阻色谱显示，YX44抗菌化合物的大小小于1000 Da。液相色谱 - 质谱能够鉴定出三种可能的候选分子，分子量分别为308、365、460和653 g/mol；这些大小均与任何已知抗生素不符。我们的结果表明，YX44似乎产生了多种具有高pH稳定性和良好溶解性的新型抗生素，对包括多重耐药菌株在内的 具有显著活性。
