Nishizawa M, Semba K, Yoshida M C, Yamamoto T, Sasaki M, Toyoshima K
Mol Cell Biol. 1986 Feb;6(2):511-7. doi: 10.1128/mcb.6.2.511-517.1986.
The nucleotide sequence of seven exons of the human c-fgr gene, a cellular homolog of the oncogene of Gardner-Rasheed feline sarcoma virus, was determined. Twenty-six independent genomic clones were obtained from a human gene library with a DNA clone of Y73 avian sarcoma virus oncogene, v-yes, as a probe under relaxed hybridization conditions. Restriction mapping and partial sequence analyses revealed that two of these clones were derived from the c-fgr gene, distinct from the c-yes gene. Interestingly, the splicing points of the c-fgr gene were identical with those of the c-src gene throughout the seven exons, suggesting that the two proto-oncogenes were generated by gene duplication of an ancestral gene containing intervening sequences. On RNA blot hybridization the major transcript was found to be 2.6 kilobase long. Two additional transcripts of 3.5 and 4.7 kilobases were also detected. Furthermore, karyotype analysis of several human-mouse hybrid cells and Southern blot analyses of DNAs of the hybrids with a human c-fgr locus-specific probe showed that this gene is located on chromosome 1.
确定了人类c-fgr基因七个外显子的核苷酸序列，该基因是加德纳-拉希德猫肉瘤病毒癌基因的细胞同源物。在宽松杂交条件下，以Y73禽肉瘤病毒癌基因v-yes的DNA克隆为探针，从人类基因文库中获得了26个独立的基因组克隆。限制性图谱分析和部分序列分析表明，其中两个克隆源自c-fgr基因，与c-yes基因不同。有趣的是，c-fgr基因的剪接位点在整个七个外显子中与c-src基因的剪接位点相同，这表明这两个原癌基因是由一个含有间隔序列的祖先基因通过基因复制产生的。在RNA印迹杂交中，发现主要转录本长度为2.6千碱基。还检测到另外两个长度分别为3.5和4.7千碱基的转录本。此外，对几种人-鼠杂交细胞的核型分析以及用人类c-fgr基因座特异性探针对杂交细胞DNA进行的Southern印迹分析表明，该基因位于1号染色体上。
