Karzbrun Eyal, Tshuva Rami Yair, Reiner Orly
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Department of Physics and the Kavli Institute for Theoretical Physics, University of California Santa Barbara, Santa Barbara, California.
Curr Protoc Cell Biol. 2018 Dec;81(1):e62. doi: 10.1002/cpcb.62. Epub 2018 Sep 21.
Brain organoids are an emerging technique for studying human neurodevelopment in vitro, with biomedical implications. However, three-dimensional tissue culture poses several challenges, including lack of nutrient exchange at the organoid core and limited imaging accessibility of whole organoids. Here we present a method for culturing organoids in a micro-fabricated device that enables in situ real-time imaging over weeks with efficient nutrient exchange by diffusion. Our on-chip approach offers a means for studying the dynamics of organoid development, cell differentiation, cell cycle, and motion. © 2018 by John Wiley & Sons, Inc.
脑类器官是一种用于体外研究人类神经发育的新兴技术，具有生物医学意义。然而，三维组织培养带来了几个挑战，包括类器官核心缺乏营养物质交换以及对整个类器官的成像可达性有限。在此，我们展示了一种在微制造装置中培养类器官的方法，该方法能够通过扩散进行有效的营养物质交换，从而在数周内进行原位实时成像。我们的芯片方法为研究类器官发育、细胞分化、细胞周期和运动的动态过程提供了一种手段。© 2018 约翰·威利父子公司。
