a Department of Medical Oncology , Huzhou Central Hospital , Huzhou , Zhejiang , China.
b Department of Intervention and Radiotherapy , Huzhou Central Hospital , Huzhou , Zhejiang Province , China.
Cancer Biol Ther. 2019;20(1):81-89. doi: 10.1080/15384047.2018.1507255. Epub 2018 Sep 21.
Abnormal lipid metabolism is considered to be one of main promoters of colorectal cancer (CRC), and intestinal microorganisms may be involved in CRC in patients with abnormal lipid metabolism.
To investigate lipid metabolism in CRC patients and explore the role of intestinal microorganisms in CRC complicated with abnormal lipid metabolism.
Overall, 150 CRC patients in Huzhou Central Hospital from January 2016 to September 2017 were recruited in the present study. Basic patient information and clinical serological indicators were investigated and analyzed. Twenty-one stool samples were collected from patients after receiving informed consent. Next-generation sequencing technology was used to sequence bacterial 16S ribosomal RNA. Bioinformatics analysis was used to profile the microbial composition and screen distinctive bacteria in patients with CRC complicated with abnormal lipid metabolism.
Apo B and FFA levels were higher in patients with stage I disease than in patients with other stages. HDL, LDL, Apo B and FFA levels were higher in female patients than in male patients. FFA level was higher in rectal cancer patients than in colon cancer patients. These differences were statistically significant (p < 0.05). The proportion of Escherichia/Shigella was increased in CRC patients with hyperlipoidaemia and hypercholesteremia; the abundance of Streptococcus was increased in CRC patients with hyperlipoidaemia; the abundance of Clostridium XIVa was reduced in CRC patients with hyperlipoidaemia and hypercholesteremia; and the abundance of Ruminococcaceae was reduced in CRC patients with hypercholesteremia. Bilophila and Butyricicoccus were closely related to CRC patients without hyperlipoidaemia or hypercholesteremia, and Selenomonas, Clostridium, Bacteroidetes Slackia, Burkholderiales and Veillonellaceae were closely related to CRC patients with hyperlipoidaemia. Some pathways, including secretion system, chaperones and folding catalysts, amino sugar and nucleotide sugar metabolism, arginine and proline metabolism, glycine, serine and threonine metabolism, histidine metabolism, pores and ion channels, nitrogen metabolism and sporulation, may be involved in lipid metabolism abnormality in CRC patients.
Many CRC patients have abnormal lipid metabolism, and the intestinal microbiota is altered in these CRC patients.
异常的脂质代谢被认为是促进结直肠癌（CRC）的主要因素之一，而肠道微生物可能参与了脂质代谢异常的 CRC 患者的发病过程。
探讨 CRC 患者的脂质代谢情况，并探索肠道微生物在伴有脂质代谢异常的 CRC 中的作用。
本研究共纳入 2016 年 1 月至 2017 年 9 月在湖州市中心医院就诊的 150 例 CRC 患者。调查并分析了患者的基本信息和临床血清学指标。采集 21 例患者知情同意后的粪便样本，采用下一代测序技术对细菌 16S 核糖体 RNA 进行测序。生物信息学分析用于分析微生物组成，并筛选伴有脂质代谢异常的 CRC 患者中具有特征性的细菌。
Ⅰ期患者的载脂蛋白 B（Apo B）和游离脂肪酸（FFA）水平高于其他分期患者。女性患者的高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、Apo B 和 FFA 水平高于男性患者。直肠癌患者的 FFA 水平高于结肠癌患者。这些差异均有统计学意义（P<0.05）。高脂血症和高胆固醇血症患者的大肠杆菌/志贺菌属比例增加；高脂血症患者的链球菌属丰度增加；高脂血症和高胆固醇血症患者的梭菌属 XIVa 减少；高胆固醇血症患者的瘤胃菌科减少。无高脂血症或高胆固醇血症的 CRC 患者与Bilophila 和 Butyricicoccus 密切相关，无高脂血症或高胆固醇血症的 CRC 患者与 Selenomonas、Clostridium、拟杆菌属 Slackia、伯克霍尔德菌目和韦荣球菌科密切相关。一些途径，包括分泌系统、伴侣和折叠催化剂、氨基酸糖和核苷酸糖代谢、精氨酸和脯氨酸代谢、甘氨酸、丝氨酸和苏氨酸代谢、组氨酸代谢、孔和离子通道、氮代谢和孢子形成，可能与 CRC 患者的脂质代谢异常有关。
许多 CRC 患者存在异常的脂质代谢，且这些 CRC 患者的肠道微生物发生改变。
