• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

嗜热四膜虫（一种真核生物）核基因组中腺嘌呤的位点特异性甲基化。

Site-specific methylation of adenine in the nuclear genome of a eucaryote, Tetrahymena thermophila.

作者信息

Harrison G S, Findly R C, Karrer K M

出版信息

Mol Cell Biol. 1986 Jul;6(7):2364-70. doi: 10.1128/mcb.6.7.2364-2370.1986.

DOI:10.1128/mcb.6.7.2364-2370.1986
PMID:3023930
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367789/
Abstract

DNA in the polyploid macronucleus of the ciliated protozoan Tetrahymena thermophila contains the modified base N6-methyladenine. We identified two GATC sites which are methylated in most or all of the 45 copies of the macronuclear genome. One site is 2 kilobases 5' to the histone H4-I gene, and the other is 5 kilobases 3' to the 73-kilodalton heat shock protein gene. These sites are de novo methylated between 10 and 16 h after initiation of conjugation, during macronuclear anlage development. The methylation states of these two GATC sites and four other unmethylated GATC sites do not change in the DNA of cells cultured under conditions which change the activity of the genes, including logarithmic growth, starvation, and heat shock.

摘要

纤毛原生动物嗜热栖热四膜虫的多倍体大核中的DNA含有修饰碱基N6-甲基腺嘌呤。我们鉴定出两个GATC位点，它们在大核基因组的45个拷贝中的大多数或全部中都被甲基化。一个位点在组蛋白H4-I基因的5'端2千碱基处，另一个位点在73千道尔顿热休克蛋白基因的3'端5千碱基处。这些位点在接合开始后10至16小时之间，在大核原基发育过程中从头甲基化。在改变基因活性的条件下培养的细胞DNA中，这两个GATC位点和其他四个未甲基化的GATC位点的甲基化状态不会改变，这些条件包括对数生长、饥饿和热休克。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af64/367789/0284ba97709c/molcellb00091-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af64/367789/2309d6006520/molcellb00091-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af64/367789/9fe70c397dbc/molcellb00091-0088-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af64/367789/e5493256f5f1/molcellb00091-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af64/367789/0284ba97709c/molcellb00091-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af64/367789/2309d6006520/molcellb00091-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af64/367789/9fe70c397dbc/molcellb00091-0088-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af64/367789/e5493256f5f1/molcellb00091-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af64/367789/0284ba97709c/molcellb00091-0090-a.jpg

相似文献

1
Site-specific methylation of adenine in the nuclear genome of a eucaryote, Tetrahymena thermophila.嗜热四膜虫（一种真核生物）核基因组中腺嘌呤的位点特异性甲基化。
Mol Cell Biol. 1986 Jul;6(7):2364-70. doi: 10.1128/mcb.6.7.2364-2370.1986.
2
Methylation of adenine in the nuclear DNA of Tetrahymena is internucleosomal and independent of histone H1.嗜热四膜虫核DNA中腺嘌呤的甲基化发生在核小体之间，且不依赖于组蛋白H1。
Nucleic Acids Res. 2002 Mar 15;30(6):1364-70. doi: 10.1093/nar/30.6.1364.
3
Methylation of ribosomal RNA genes in the macronucleus of Tetrahymena thermophila.嗜热四膜虫大核中核糖体RNA基因的甲基化
Nucleic Acids Res. 1983 Aug 11;11(15):5131-45. doi: 10.1093/nar/11.15.5131.
4
Molecular analysis of N6-methyladenine patterns in Tetrahymena thermophila nuclear DNA.嗜热四膜虫核DNA中N6-甲基腺嘌呤模式的分子分析。
Mol Cell Biol. 1989 Jun;9(6):2598-605. doi: 10.1128/mcb.9.6.2598-2605.1989.
5
Position effect takes precedence over target sequence in determination of adenine methylation patterns in the nuclear genome of a eukaryote, Tetrahymena thermophila.在真核生物嗜热栖热放线菌的核基因组中，位置效应在腺嘌呤甲基化模式的决定中优先于靶序列。
Nucleic Acids Res. 1998 Oct 15;26(20):4566-73. doi: 10.1093/nar/26.20.4566.
6
Position effect for adenine methylation in the macronuclear DNA of Tetrahymena.嗜热四膜虫大核DNA中腺嘌呤甲基化的位置效应
Gene. 1995 May 19;157(1-2):235-7. doi: 10.1016/0378-1119(94)00546-5.
7
DNA synthesis, methylation and degradation during conjugation in Tetrahymena thermophila.嗜热四膜虫接合过程中的DNA合成、甲基化及降解
Nucleic Acids Res. 1985 Jan 11;13(1):73-87. doi: 10.1093/nar/13.1.73.
8
Tetrahymena H4 genes: structure, evolution and organization in macro- and micronuclei.嗜热四膜虫H4基因：在大核和小核中的结构、进化及组织形式
Nucleic Acids Res. 1984 Feb 24;12(4):1961-75. doi: 10.1093/nar/12.4.1961.
9
Constancy of adenine methylation in Tetrahymena macronuclear DNA.嗜热四膜虫大核DNA中腺嘌呤甲基化的稳定性
J Protozool. 1990 Sep-Oct;37(5):409-14. doi: 10.1111/j.1550-7408.1990.tb01165.x.
10
Formation of stable chromatin structures on the histone H4 gene during differentiation in Tetrahymena thermophila.嗜热四膜虫分化过程中组蛋白H4基因上稳定染色质结构的形成。
Mol Cell Biol. 1986 Aug;6(8):3014-7. doi: 10.1128/mcb.6.8.3014-3017.1986.

引用本文的文献

1
Case Study of the Response of N-Methyladenine DNA Modification to Environmental Stressors in the Unicellular Eukaryote Tetrahymena thermophila.单细胞真核生物嗜热四膜虫中 N-甲基腺嘌呤 DNA 修饰对环境胁迫的响应的案例研究。
mSphere. 2021 Jun 30;6(3):e0120820. doi: 10.1128/mSphere.01208-20. Epub 2021 May 28.
2
Biodiversity-based development and evolution: the emerging research systems in model and non-model organisms.基于生物多样性的发展与进化：模型和非模型生物新兴研究体系。
Sci China Life Sci. 2021 Aug;64(8):1236-1280. doi: 10.1007/s11427-020-1915-y. Epub 2021 Apr 22.
3
Programmed genome rearrangements in ciliates.

本文引用的文献

1
PROPERTIES OF THE SATELLITE DNA ASSOCIATED WITH THE CHLOROPLASTS OF EUGLENA GRACILIS.与纤细裸藻叶绿体相关的卫星DNA的特性
J Mol Biol. 1964 Sep;9:812-24. doi: 10.1016/s0022-2836(64)80187-x.
2
Deoxyribonucleic acid methylation and chromatin organization in Tetrahymena thermophila.嗜热四膜虫中的脱氧核糖核酸甲基化与染色质组织
Mol Cell Biol. 1981 Jul;1(7):600-8. doi: 10.1128/mcb.1.7.600-608.1981.
3
Chromatin structure of the ribosomal RNA genes of Tetrahymena thermophila as analyzed by trimethylpsoralen crosslinking in vivo.
纤毛虫中的程序性基因组重排。
Cell Mol Life Sci. 2020 Nov;77(22):4615-4629. doi: 10.1007/s00018-020-03555-2. Epub 2020 May 27.
4
N6-adenine DNA methylation is associated with the linker DNA of H2A.Z-containing well-positioned nucleosomes in Pol II-transcribed genes in Tetrahymena.N6-腺嘌呤DNA甲基化与嗜热四膜虫中Pol II转录基因中含H2A.Z的定位良好的核小体的连接DNA相关。
Nucleic Acids Res. 2017 Nov 16;45(20):11594-11606. doi: 10.1093/nar/gkx883.
5
Nucleic Acid Modifications in Regulation of Gene Expression.核酸修饰在基因表达调控中的作用。
Cell Chem Biol. 2016 Jan 21;23(1):74-85. doi: 10.1016/j.chembiol.2015.11.007.
6
N6-methyladenine: the other methylated base of DNA.N6-甲基腺嘌呤：DNA的另一种甲基化碱基。
Bioessays. 2006 Mar;28(3):309-15. doi: 10.1002/bies.20342.
7
Methylation of adenine in the nuclear DNA of Tetrahymena is internucleosomal and independent of histone H1.嗜热四膜虫核DNA中腺嘌呤的甲基化发生在核小体之间，且不依赖于组蛋白H1。
Nucleic Acids Res. 2002 Mar 15;30(6):1364-70. doi: 10.1093/nar/30.6.1364.
8
Methylation site within a facultatively persistent sequence in the macronucleus of Tetrahymena thermophila.嗜热四膜虫大核中一个兼性持久序列内的甲基化位点。
Mol Cell Biol. 1986 Dec;6(12):4742-4. doi: 10.1128/mcb.6.12.4742-4744.1986.
9
Transformation of Tetrahymena thermophila with hypermethylated rRNA genes.嗜热四膜虫被高度甲基化的核糖体RNA基因转化。
Mol Cell Biol. 1988 Apr;8(4):1664-9. doi: 10.1128/mcb.8.4.1664-1669.1988.
10
A family of DNA sequences is reproducibly rearranged in the somatic nucleus of Tetrahymena.在四膜虫的体细胞核中，一个DNA序列家族可重复性地发生重排。
Nucleic Acids Res. 1986 Oct 24;14(20):8007-25. doi: 10.1093/nar/14.20.8007.
通过体内三甲基补骨脂素交联分析嗜热四膜虫核糖体RNA基因的染色质结构。
J Mol Biol. 1980 Feb 5;136(4):395-416. doi: 10.1016/0022-2836(80)90397-6.
4
Identification and purification of young macronuclear anlagen from conjugating cells of Tetrahymena thermophila.从嗜热四膜虫接合细胞中鉴定和纯化幼龄大核原基。
Dev Biol. 1982 Oct;93(2):519-33. doi: 10.1016/0012-1606(82)90139-7.
5
Conjugation in Tetrahymena thermophila. A temporal analysis of cytological stages.嗜热四膜虫中的接合作用。细胞学阶段的时间分析。
Exp Cell Res. 1982 Jul;140(1):227-36. doi: 10.1016/0014-4827(82)90172-0.
6
Sequence specificity of DNA adenine methylase in the protozoan Tetrahymena thermophila.嗜热四膜虫中DNA腺嘌呤甲基化酶的序列特异性
J Bacteriol. 1982 May;150(2):993-6. doi: 10.1128/jb.150.2.993-996.1982.
7
Methylated bases in the DNA of the ciliate Stylonychia mytilus.
Eur J Cell Biol. 1981 Apr;24(1):154-6.
8
Germ line-specific DNA sequences are present on all five micronuclear chromosomes in Tetrahymena thermophila.种系特异性DNA序列存在于嗜热四膜虫的所有五条微核染色体上。
Mol Cell Biol. 1983 Nov;3(11):1909-19. doi: 10.1128/mcb.3.11.1909-1919.1983.
9
Starved Tetrahymena thermophila cells that are unable to mount an effective heat shock response selectively degrade their rRNA.无法产生有效热休克反应的饥饿嗜热四膜虫细胞会选择性地降解其核糖体RNA。
Mol Cell Biol. 1984 Oct;4(10):2170-9. doi: 10.1128/mcb.4.10.2170-2179.1984.
10
Timing of the appearance of macronuclear-specific histone variant hv1 and gene expression in developing new macronuclei of Tetrahymena thermophila.嗜热四膜虫发育中新大核中巨核特异性组蛋白变体hv1的出现时间及基因表达
J Cell Biol. 1984 Jun;98(6):2107-17. doi: 10.1083/jcb.98.6.2107.