Laboratory of Biothecnological Process, Antibiotics Department, Federal University of Pernambuco, Pernambuco, Brazil.
Laboratory of Immunological and Antitumor Analyzes, Antibiotics Department, Federal University of Pernambuco, Pernambuco, Brazil.
Int J Biol Macromol. 2019 Feb 15;123:1331-1339. doi: 10.1016/j.ijbiomac.2018.09.120. Epub 2018 Sep 21.
Opuntia fícus-indica and Opuntia cochenillifera are species of Cactaceae, found in the arid regions of the planet. They present water, cellulose, hemicellulose, pectins, extractives, ashes and lignins. Here we aimed to study the immunomodulatory action of lignins from these two species against mice splenocytes, since no study for this purpose has yet been reported. The antioxidant activities of these lignins were evaluated by the DPPH, ABTS, NO assays and total antioxidant activity. Cytotoxicity was evaluated through Annexin V-FITC and propidium iodide-PE probs and cell proliferation was determined by CFSE. Immunomodulation studies with Opuntia lignins obtained were performed through investigation of ROS levels, cytosolic calcium release, changes on mitochondrial membrane potential, cytokine production and NO release. Results showed that Opuntia cochenillifera lignin presented more phenolic amount and antioxidant activities than Opuntia ficius-indica. Both lignins showed high cell viability (>96%) and cell proliferation. Activation signal was observed for both lignins with increase of ROS and cytosolic calcium levels, and changes in mitochondrial membrane potential. In addition, lignins induced high TNF-α, IL-6 and IL-10 production and reduced NO release. Therefore, these lignins present great potential to be used as molecules with a proinflammatory profile, being shown as a promising therapeutic agent.
仙人掌果和胭脂仙人掌是仙人掌科的两种植物，分布在地球上的干旱地区。它们含有水分、纤维素、半纤维素、果胶、浸出物、灰分和木质素。在这里，我们旨在研究这两种植物木质素对小鼠脾细胞的免疫调节作用，因为目前尚无为此目的而进行的研究。通过 DPPH、ABTS、NO 测定法和总抗氧化活性评估这些木质素的抗氧化活性。通过 Annexin V-FITC 和碘化丙啶-PE 探针评估细胞毒性，通过 CFSE 测定细胞增殖。通过研究 ROS 水平、细胞质钙释放、线粒体膜电位变化、细胞因子产生和 NO 释放来进行 Opuntia 木质素的免疫调节研究。结果表明，胭脂仙人掌木质素的酚类物质含量和抗氧化活性均高于仙人掌果木质素。两种木质素均表现出较高的细胞活力（>96%）和细胞增殖。两种木质素均观察到激活信号，表现为 ROS 和细胞质钙水平升高，以及线粒体膜电位变化。此外，木质素诱导产生高 TNF-α、IL-6 和 IL-10 并减少 NO 释放。因此，这些木质素具有作为具有促炎特性的分子的巨大潜力，有望成为一种有前途的治疗剂。
