Moran E, Zerler B, Harrison T M, Mathews M B
Mol Cell Biol. 1986 Oct;6(10):3470-80. doi: 10.1128/mcb.6.10.3470-3480.1986.
The transformation and early adenovirus gene transactivation functions of the E1A region were analyzed with deletion and point mutations. Deletion of amino acids from position 86 through 120 had little effect on the lytic or transforming functions of the E1A products, while deletion of amino acids from position 121 through 150 significantly impaired both functions. The sensitivity of the transformation function to alterations in the region from amino acid position 121 to 150 was further indicated by the impairment of transforming activity resulting from single amino acid substitutions at positions 124 and 135. Interestingly, conversion of a cysteine residue at position 124 to glycine severely impaired the transformation function without affecting the early adenovirus gene activating functions. Single amino acid substitutions in a different region of the E1A gene had the converse effect. All the mutants produced polypeptides of sufficient stability to be detected by Western immunoblot analysis. The single amino acid substitutions at positions 124 and 135, although impairing the transformation functions, did not detectably alter the formation of the higher-apparent-molecular-weight forms of the E1A products.
通过缺失和点突变分析了E1A区域的转化和早期腺病毒基因反式激活功能。从第86位至120位氨基酸的缺失对E1A产物的裂解或转化功能影响很小，而从第121位至150位氨基酸的缺失则显著损害了这两种功能。第124位和135位单个氨基酸替换导致转化活性受损，进一步表明转化功能对第121位至150位区域变化的敏感性。有趣的是，第124位的半胱氨酸残基转换为甘氨酸严重损害了转化功能，而不影响早期腺病毒基因激活功能。E1A基因不同区域的单个氨基酸替换则产生相反的效果。所有突变体产生的多肽稳定性足以通过蛋白质免疫印迹分析检测到。第124位和135位的单个氨基酸替换虽然损害了转化功能，但并未显著改变E1A产物较高表观分子量形式的形成。
