Zerler B, Moran B, Maruyama K, Moomaw J, Grodzicker T, Ruley H E
Mol Cell Biol. 1986 Mar;6(3):887-99. doi: 10.1128/mcb.6.3.887-899.1986.
Plasmids expressing partial adenovirus early region 1A (E1A) coding sequences were tested for activities which facilitate in vitro establishment (immortalization) of primary baby rat kidney cells and which enable the T24 Harvey ras-related oncogene and the polyomavirus middle T antigen (pmt) gene to transform primary baby rat kidney cells. E1A cDNAs expressing the 289- and 243-amino acid proteins expressed both E1A transforming functions. Mutant hrA, which encodes a 140-amino acid protein derived from the amino-terminal domain shared by the 289- and 243-amino acid proteins, enabled ras (but not pmt) to transform and facilitated in vitro establishment to a low, but detectable, extent. These studies suggest that E1A functions which collaborate with ras oncogenes and those which facilitate establishment are linked. Furthermore, E1A transforming functions are not associated with activities of the 289-amino acid E1A proteins required for efficient transcriptional activation of viral early region promoters.
对表达部分腺病毒早期区域1A（E1A）编码序列的质粒进行了活性测试，这些活性有助于原代新生大鼠肾细胞的体外建立（永生化），并使T24哈维ras相关癌基因和多瘤病毒中T抗原（pmt）基因能够转化原代新生大鼠肾细胞。表达289和243个氨基酸蛋白的E1A cDNA均表达了E1A的转化功能。突变体hrA编码一种140个氨基酸的蛋白，该蛋白来源于289和243个氨基酸蛋白共有的氨基末端结构域，它能使ras（但不能使pmt）发生转化，并在低但可检测的程度上促进体外建立。这些研究表明，与ras癌基因协同作用的E1A功能和促进细胞建立的功能是相关联的。此外，E1A的转化功能与病毒早期区域启动子有效转录激活所需的289个氨基酸E1A蛋白的活性无关。
