Coincident gene conversion events in yeast that involve a large insertion.
作者信息
Golin J E, Falco S C, Margolskee J P
出版信息
Genetics. 1986 Dec;114(4):1081-94. doi: 10.1093/genetics/114.4.1081.
Abstract
In yeast, spontaneous gene conversion events involving sites that are far apart (16 cM) occur 1000 times more frequently in mitotic cells than is expected for two independent acts of recombination. It has been proposed that a major portion of these could be due to a long, continuous heteroduplex intermediate. We have examined this possibility in further detail by introducing, via transformation, a large plasmid insertion between the LEU1 and TRP5 loci and studying its behavior among coincident convertants involving the flanking sites. Among such convertants, there is frequent loss of the plasmid when it is present in hemizygous or homozygous configuration. Our results could support the long heteroduplex model for coincident recombination events, but only if novel assumptions regarding the formation and fate of mismatched DNA are made. Therefore, an alternative model that proposes multiple, concerted recombination events is discussed.
摘要
相似文献
1
Coincident gene conversion events in yeast that involve a large insertion.
Genetics. 1986 Dec;114(4):1081-94. doi: 10.1093/genetics/114.4.1081.
2
The behavior of insertions near a site of mitotic gene conversion in yeast.
Genetics. 1988 Jul;119(3):535-40. doi: 10.1093/genetics/119.3.535.
3
Coincident gene conversion during mitosis in saccharomyces.
Genetics. 1984 Jul;107(3):355-65. doi: 10.1093/genetics/107.3.355.
4
Frequencies of mutagen-induced coincident mitotic recombination at unlinked loci in Saccharomyces cerevisiae.
Mutat Res. 2007 Mar 1;616(1-2):119-32. doi: 10.1016/j.mrfmmm.2006.11.014. Epub 2006 Dec 6.
5
Long-tract mitotic gene conversion in yeast: evidence for a triparental contribution during spontaneous recombination.
Genetics. 1994 Jun;137(2):439-53. doi: 10.1093/genetics/137.2.439.
6
Coincident recombination during mitosis in saccharomyces: distance-dependent and -independent components.
Genetics. 1988 Jul;119(3):541-7. doi: 10.1093/genetics/119.3.541.
7
8
Comparative analysis of spontaneous mitotic recombination in [cir0] and [cir+] strains of the yeast Saccharomyces cerevisiae.
Curr Genet. 1992 Oct;22(4):259-65. doi: 10.1007/BF00317918.
9
Mitotic and meiotic gene conversion of Ty elements and other insertions in Saccharomyces cerevisiae.
Genetics. 1989 Aug;122(4):759-72. doi: 10.1093/genetics/122.4.759.
10
Induction of recombination between homologous and diverged DNAs by double-strand gaps and breaks and role of mismatch repair.
Mol Cell Biol. 1994 Jul;14(7):4802-14. doi: 10.1128/mcb.14.7.4802-4814.1994.
引用本文的文献
1
Intrachromosomal recombination between well-separated, homologous sequences in mammalian cells.
Genetics. 1999 Jun;152(2):685-97. doi: 10.1093/genetics/152.2.685.
2
Differential mismatch repair can explain the disproportionalities between physical distances and recombination frequencies of cyc1 mutations in yeast.
Genetics. 1988 May;119(1):21-34. doi: 10.1093/genetics/119.1.21.
3
Mitotic sectored colonies: evidence of heteroduplex DNA formation during direct repeat recombination.
Proc Natl Acad Sci U S A. 1988 Apr;85(8):2696-700. doi: 10.1073/pnas.85.8.2696.
4
A reexamination of the role of the RAD52 gene in spontaneous mitotic recombination.
Curr Genet. 1988 Sep;14(3):211-23. doi: 10.1007/BF00376741.
5
Coincident recombination during mitosis in saccharomyces: distance-dependent and -independent components.
Genetics. 1988 Jul;119(3):541-7. doi: 10.1093/genetics/119.3.541.
6
The behavior of insertions near a site of mitotic gene conversion in yeast.
Genetics. 1988 Jul;119(3):535-40. doi: 10.1093/genetics/119.3.535.
7
Analysis of the mechanism for reversion of a disrupted gene.
Genetics. 1988 Jun;119(2):237-47. doi: 10.1093/genetics/119.2.237.
8
Physical lengths of meiotic and mitotic gene conversion tracts in Saccharomyces cerevisiae.
Genetics. 1988 Mar;118(3):401-10. doi: 10.1093/genetics/118.3.401.
9
Gene targeting with retroviral vectors: recombination by gene conversion into regions of nonhomology.
Mol Cell Biol. 1989 Apr;9(4):1621-7. doi: 10.1128/mcb.9.4.1621-1627.1989.
10
Cloning by gene amplification of two loci conferring multiple drug resistance in Saccharomyces.
Genetics. 1990 May;125(1):13-20. doi: 10.1093/genetics/125.1.13.
本文引用的文献
1
Mitotic recombination: mismatch correction and replicational resolution of Holliday structures formed at the two strand stage in Saccharomyces.
Mol Gen Genet. 1981;183(2):252-63. doi: 10.1007/BF00270626.
2
Hybrid DNA formation during meiotic recombination.
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7648-51. doi: 10.1073/pnas.78.12.7648.
3
Gene conversion and associated reciprocal recombination are separable events in vegetative cells of Saccharomyces cerevisiae.
Proc Natl Acad Sci U S A. 1983 Nov;80(22):6912-6. doi: 10.1073/pnas.80.22.6912.
4
DNA rearrangements associated with a transposable element in yeast.
Cell. 1980 Aug;21(1):239-49. doi: 10.1016/0092-8674(80)90131-2.
5
Effects of nonhomology on bacteriophage lambda recombination.
Genetics. 1983 Jan;103(1):5-22. doi: 10.1093/genetics/103.1.5.
6
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
7
Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.
Gene. 1979 Dec;8(1):17-24. doi: 10.1016/0378-1119(79)90004-0.
8
Replacement of chromosome segments with altered DNA sequences constructed in vitro.
Proc Natl Acad Sci U S A. 1979 Oct;76(10):4951-5. doi: 10.1073/pnas.76.10.4951.
9
Evidence that spontaneous mitotic recombination occurs at the two-strand stage.
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4436-40. doi: 10.1073/pnas.75.9.4436.
10
Evidence for joint genic control of spontaneous mutation and genetic recombination during mitosis in Saccharomyces.
Mol Gen Genet. 1977 Jan 18;150(2):127-35. doi: 10.1007/BF00695392.
Zotero 插件