Rahimi Maryam, Ghoreshi Mina, Emami Bahman, Shafei Mohammad Naser, Hosseini Mahmoud, Khajavirad Abolfazl
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Int J Prev Med. 2018 Oct 12;9:92. doi: 10.4103/ijpvm.IJPVM_312_17. eCollection 2018.
(R.D) is an aromatic plant with numerous therapeutic effects including cardiovascular effect. The mechanism cardiovascular effect of R.D is unclear and suggested mediated through renin-angiotensin system (RAS). Therefore, in this study, the role of hydroalcoholic extract of R.D on acute hypertension induced by AngII was evaluated.
After anesthesia, femoral artery and vein of rats were cannulated for recording cardiovascular responses and drug injection, respectively. Systolic blood pressure (SBP), mean arterial blood pressure (MAP), and heart rate (HR) were recorded continuously by power lab software. Rats were divided into saline, AngII (50 ng/kg), AngII + Losartan (10 mg/kg), and three groups of R.D extract (250, 500, and 1000 mg/kg). Losartan and AngII were administered intravenously and the other ones intraperitoneal. In the R.D groups, 30 min after injection of the extract, AngII was injected and the maximum changes in SBP, MAP, and HR were calculated and compared to that in control and AngII groups.
Results show that AngII significantly increased SBP, MAP, and decreased HR than the control group which was blocked by losartan. SBP and MAP in R.D + AngII groups were significantly lower than AngII alone ( < 0.05 - < 0.001). Only MAP in higher dose (1000 mg/kg) was significantly lower than low dose (250 mg/kg; < 0.05). Two higher doses also significantly decreased bradycardia induced by AngII ( < 0. 01).
The preventive effect of hydroalcoholic extract of R.D on cardiovascular parameters maybe is mediated by suppression of AngII activity.
RD是一种具有多种治疗作用的芳香植物，包括心血管方面的作用。RD的心血管作用机制尚不清楚，推测是通过肾素-血管紧张素系统（RAS）介导的。因此，在本研究中，评估了RD水醇提取物对AngII诱导的急性高血压的作用。
麻醉后，分别将大鼠的股动脉和静脉插管，用于记录心血管反应和注射药物。通过PowerLab软件连续记录收缩压（SBP）、平均动脉压（MAP）和心率（HR）。将大鼠分为生理盐水组、AngII（50 ng/kg）组、AngII + 氯沙坦（10 mg/kg）组和三组RD提取物组（250、500和1000 mg/kg）。氯沙坦和AngII通过静脉注射给药，其他通过腹腔注射给药。在RD组中，注射提取物30分钟后，注射AngII，并计算SBP、MAP和HR的最大变化，并与对照组和AngII组进行比较。
结果显示，与对照组相比，AngII显著升高SBP、MAP并降低HR，而氯沙坦可阻断这种作用。RD + AngII组的SBP和MAP显著低于单独使用AngII组（< 0.05 - < 0.001）。仅高剂量（1000 mg/kg）的MAP显著低于低剂量（250 mg/kg；< 0.05）。两个较高剂量也显著降低了AngII诱导的心动过缓（< 0.01）。
RD水醇提取物对心血管参数的预防作用可能是通过抑制AngII活性介导的。
