Iwahashi Yumiko
National Agriculture and Food Research Organization, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8642, Japan.
AMB Express. 2018 Oct 3;8(1):161. doi: 10.1186/s13568-018-0688-y.
Fusarium species are plant pathogens that produce various mycotoxins. Here, the regulatory mechanism of deoxynivalenol production in Fusarium asiaticum was analyzed using proteomic, metabolomic and transcriptomic methods. F. asiaticum was induced to produce deoxynivalenol by adding agmatine to the culture medium. Subsequently, metabolites of the glycolysis system were increased but mRNAs of the corresponding proteins were not up regulated. We speculated that this phenomenon was due to the up regulation of the 6-fructokinase and pyruvate kinase proteins, which are key enzymes of glycolysis. We discuss the relationship of metabolism with the regulation of deoxynivalenol production in F. asiaticum.
镰刀菌属是产生多种霉菌毒素的植物病原体。在此，利用蛋白质组学、代谢组学和转录组学方法分析了亚洲镰刀菌中脱氧雪腐镰刀菌烯醇的产生调控机制。通过向培养基中添加胍丁胺诱导亚洲镰刀菌产生脱氧雪腐镰刀菌烯醇。随后，糖酵解系统的代谢产物增加，但相应蛋白质的mRNA未上调。我们推测这种现象是由于糖酵解的关键酶6-果糖激酶和丙酮酸激酶蛋白的上调所致。我们讨论了亚洲镰刀菌中代谢与脱氧雪腐镰刀菌烯醇产生调控的关系。
