Institute for Polymers, Composites and Biomaterials, National Research Council of Italy, 80078 Pozzuoli, Italy.
Institute of Agro-environmental and Forest Biology, National Research Council of Italy, 80131 Naples, Italy.
Biosensors (Basel). 2018 Oct 4;8(4):87. doi: 10.3390/bios8040087.
In the present contribution the fabrication and characterization of functionalized gold nanospheres of uniform shape and controlled size is reported. These nano-objects are intended to be used as Surface Enhanced Raman Spectroscopy (SERS) sensors for cellular uptake and localization. Thiophenol was used as molecular reporter and was bound to the Au surface by a chemisorption process in aqueous solution. The obtained colloidal solution was highly stable and no aggregation of the single nanospheres into larger clusters was observed. The nanoparticles were incubated in human prostatic cells with the aim of developing a robust, SERS-based method to differentiate normal and tumor cell lines. SERS imaging experiments showed that tumor cells uptake considerably larger amounts of nanoparticles in comparison to normal cells (up to 950% more); significant differences were also observed in the uptake kinetics. This largely different behaviour might be exploited in diagnostic and therapeutic applications.
本研究报告了功能化金纳米球的制备和表征，这些纳米球具有均匀的形状和可控的尺寸，旨在用作细胞摄取和定位的表面增强拉曼光谱（SERS）传感器。噻吩酚被用作分子报告物，并通过在水溶液中的化学吸附过程结合到 Au 表面上。所得胶体溶液非常稳定，未观察到单个纳米球聚集成更大的簇。将纳米颗粒孵育在人前列腺细胞中，旨在开发一种基于 SERS 的稳健方法，以区分正常和肿瘤细胞系。SERS 成像实验表明，与正常细胞相比，肿瘤细胞摄取的纳米颗粒数量要多得多（高达 950%）；在摄取动力学方面也观察到显著差异。这种截然不同的行为可能在诊断和治疗应用中得到利用。
