Wainberg M A, Portnoy J, Tsoukas C, Gilmore N
Immunology. 1987 Feb;60(2):275-80.
Peripheral blood lymphocytes of patients with AIDS are generally anergic to stimulation by lectins or by alloantigens. We have succeeded in demonstrating that the lymphocytes of six AIDS patients, with histories of herpes simplex virus (HSV)-induced genital, anal and/or oral lesions, retained functional specificity with regard to HSV antigens. This was accomplished by treating patient lymphocytes with OKT8 monoclonal antibodies, in the presence of complement, to yield a cell population that was partially responsive to phytohaemagglutinin (PHA). Enhanced proliferation was obtained in the presence of exogenous interleukin-2, which was also employed to foster the growth of these cells over several generations. Lymphocytes derived from these AIDS patients, and maintained in vitro as described, were specifically stimulated in proliferation assays by a HSV antigen preparation in six of the ten cases studied.
艾滋病患者的外周血淋巴细胞通常对凝集素或同种异体抗原的刺激无反应。我们成功地证明，6名有单纯疱疹病毒（HSV）引起的生殖器、肛门和/或口腔病变病史的艾滋病患者的淋巴细胞，对HSV抗原保持功能特异性。这是通过在补体存在的情况下，用OKT8单克隆抗体处理患者淋巴细胞，以产生对植物血凝素（PHA）有部分反应的细胞群体来实现的。在外源白细胞介素-2存在的情况下可获得增强的增殖，白细胞介素-2也用于促进这些细胞几代的生长。在10例研究病例中的6例中，来自这些艾滋病患者并按上述方法在体外维持的淋巴细胞，在增殖试验中被HSV抗原制剂特异性刺激增殖。
