大肠杆菌gyrA和gyrB调控区与半乳糖激酶基因的融合体可通过香豆霉素处理诱导表达。
Fusions of the Escherichia coli gyrA and gyrB control regions to the galactokinase gene are inducible by coumermycin treatment.
作者信息
Menzel R, Gellert M
出版信息
J Bacteriol. 1987 Mar;169(3):1272-8. doi: 10.1128/jb.169.3.1272-1278.1987.
Abstract
We have previously shown that the genes encoding the two subunits of Escherichia coli DNA gyrase are regulated in a manner which is dependent on DNA conformation. When the DNA encoding the gyrA and gyrB genes is relaxed, both genes are expressed at a high level; in negatively supercoiled DNA they are expressed at a low level. In this paper we describe fusions of both the gyrA and gyrB 5' sequences to the E. coli galactokinase gene. In such fusions we found that galactokinase can be induced by treating the cells with coumermycin A1, an inhibitor of DNA gyrase. Our results suggest that the regulation occurs at the transcriptional level and that only a small region of DNA is necessary for coumermycin-induced gene expression.
摘要
我们之前已经表明，编码大肠杆菌DNA促旋酶两个亚基的基因是以一种依赖于DNA构象的方式进行调控的。当编码gyrA和gyrB基因的DNA处于松弛状态时，这两个基因都高水平表达；在负超螺旋DNA中，它们则低水平表达。在本文中，我们描述了gyrA和gyrB 5'序列与大肠杆菌半乳糖激酶基因的融合。在这种融合中，我们发现用香豆霉素A1（一种DNA促旋酶抑制剂）处理细胞可以诱导半乳糖激酶的表达。我们的结果表明，这种调控发生在转录水平，并且香豆霉素诱导的基因表达仅需要一小段DNA区域。
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