Graduate School of Medical Technology, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo, 173-8605, Japan.
Department of Clinical Laboratory, Saiseikai Utsunomiya Hospital, 911-1 Takebayashi-machi, Utsunomiya, Tochigi, 391-0974, Japan.
Mycopathologia. 2019 Feb;184(1):13-21. doi: 10.1007/s11046-018-0299-9. Epub 2018 Oct 5.
Aspergillus species are the most common pathogenic fungi involved in otomycosis, an infection of the outer ear canal. In this study, we examined the incidence of Aspergillus infections and the antifungal susceptibilities of 30 Aspergillus species isolates from patients with otomycosis who visited Saiseikai Utsunomiya Hospital between August 2013 and July 2016. Based on the morphological test results, the strains were identified as Aspergillus niger sensu lato (20 strains), A. terreus sensu lato (7 strains), and A. fumigatus sensu lato (3 strains). In contrast, the molecular identifications based on analyzing the isolates' partial β-tubulin gene sequences revealed them to be A. niger sensu stricto (12 strains), A. tubingensis (8 strains), A. terreus sensu stricto (7 strains), and A. fumigatus sensu stricto (3 strains). The antifungal susceptibility test results indicated that strains of A. tubingensis and A. niger sensu stricto displayed lower susceptibilities to ravuconazole, compared with the other isolates. The Aspergillus strains from this study showed low minimum inhibitory concentrations toward the azole-based drugs efinaconazole, lanoconazole, and luliconazole. Therefore, these topical therapeutic agents may be effective for the treatment of otomycosis.
曲霉菌属是最常见的致病性真菌，与外耳道感染（耳霉菌病）有关。本研究于 2013 年 8 月至 2016 年 7 月期间，调查了 30 株来我院就诊的耳霉菌病患者分离的曲霉菌属感染情况及其对 30 株曲霉菌属分离株的抗真菌药敏性。根据形态学检测结果，这些菌株被鉴定为黑曲霉（20 株）、土曲霉（7 株）和烟曲霉（3 株）。相比之下，基于分析分离株部分β-微管蛋白基因序列的分子鉴定结果显示，这些菌株为严格意义上的黑曲霉（12 株）、构巢曲霉（8 株）、土曲霉（7 株）和烟曲霉（3 株）。抗真菌药敏试验结果表明，与其他分离株相比，构巢曲霉和严格意义上的黑曲霉对拉夫康唑的敏感性较低。本研究中的曲霉菌株对唑类药物依氟康唑、酮康唑和卢立康唑的最低抑菌浓度较低。因此，这些局部治疗药物可能对耳霉菌病的治疗有效。
