Charannya Seetharam, Duraivel Dasarathan, Padminee Krishnan, Poorni Saravanan, Nishanthine Cruz, Srinivasan Manali Ramakrishnan
Department of Conservative Dentistry and Endodontics, Sri Venkateswara Dental College and Hospital, Chennai, Tamil Nadu, India.
Contemp Clin Dent. 2018 Sep;9(Suppl 2):S204-S209. doi: 10.4103/ccd.ccd_869_17.
Silver nanoparticle (AgNP) is a potent antimicrobial that is widely used in several fields of medicine. Chlorhexidine (CHX) gluconate is a well-known agent used in dentistry to eliminate oral microbial flora.
The aim of this study is to evaluate the efficacy of AgNPs, 2% CHX gluconate, and the combination of two solutions against endodontic pathogens such as , , and . These organisms are frequently found in the root canal space and their persistence may lead to endodontic failure. The synergistic effect of the two solutions has been evaluated in this study. The antibiotic gentamycin was taken as the control group.
Agar well diffusion method was used and minimum inhibitory concentration of AgNP was found to be 15 μg/mL. AgNPs were synthesized from the aqueous plant extract of . The combination of CHX-AgNP solution was stirred together by a glass rod. The values were tabulated and subjected to statistical analysis using the SPSS software version 20. One-way ANOVA test was used to compare within the groups and between groups. The level of significance was set at 5%.
CHX-AgNP combined solution exhibited the highest efficacy in comparison to these solutions used alone. They showed the highest efficacy against among the three organisms tested.
The present study demonstrates the antimicrobial efficacy of a novel mixture of CHX-AgNP solution, and it may be developed as a promising antimicrobial agent against endodontic flora.
银纳米颗粒（AgNP）是一种有效的抗菌剂，广泛应用于多个医学领域。葡萄糖酸氯己定（CHX）是牙科中用于消除口腔微生物群的知名药剂。
本研究的目的是评估AgNP、2%葡萄糖酸氯己定以及两种溶液的组合对牙髓病原体如[具体病原体名称1]、[具体病原体名称2]和[具体病原体名称3]的疗效。这些微生物经常在根管空间中被发现，它们的持续存在可能导致牙髓治疗失败。本研究评估了两种溶液的协同作用。抗生素庆大霉素作为对照组。
采用琼脂扩散法，发现AgNP的最低抑菌浓度为15μg/mL。AgNP由[植物名称]的水提取物合成。用玻璃棒将CHX-AgNP溶液搅拌在一起。将数值列表，并使用SPSS 20版软件进行统计分析。采用单因素方差分析在组内和组间进行比较。显著性水平设定为5%。
与单独使用这些溶液相比，CHX-AgNP混合溶液表现出最高的疗效。在测试的三种微生物中，它们对[具体病原体名称]显示出最高的疗效。
本研究证明了CHX-AgNP溶液新型混合物的抗菌效果，它可能被开发成为一种有前景的抗牙髓菌群抗菌剂。
