• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

多核苷酸的固相合成。IV. 磷酸三酯法中聚苯乙烯树脂在聚脱氧核糖核苷酸合成中的应用。

Solid-phase synthesis of polynucleotides. IV. Usage of polystyrene resins for the synthesis of polydeoxyribonucleotides by the phosphostriester method.

作者信息

Miyoshi K, Arentzen R, Huang T, Itakura K

出版信息

Nucleic Acids Res. 1980 Nov 25;8(22):5507-17. doi: 10.1093/nar/8.22.5507.

DOI:10.1093/nar/8.22.5507
PMID:7465420
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324317/
Abstract

Contrary to the expectation, the Merrifield polystyrene resin, 2% cross-linked by divinylbenzene, is as efficient as the polyacrylmorpholide resin for the synthesis of polydeoxyribonucleotides using a phosphotriester method. On the Merrifield resin, the tetradecamer, dTpCpGpTpCpApApCpTpGpGpCpTpT, and the hexadecamer, dCpCpApGpTpCpApCpGpApCpGpTpTpGpT, were synthesized by the phosphotriester method using di and trinucleotide blocks as coupling units.

摘要

与预期相反，用二乙烯基苯交联2%的 Merrifield 聚苯乙烯树脂在使用磷酸三酯法合成聚脱氧核糖核苷酸时与聚丙烯酰吗啉树脂一样有效。在 Merrifield 树脂上，使用二核苷酸和三核苷酸片段作为偶联单元，通过磷酸三酯法合成了十四聚体 dTpCpGpTpCpApApCpTpGpGpCpTpT 和十六聚体 dCpCpApGpTpCpApCpGpApCpGpTpTpGpT。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b8f/324317/fa3c09a9338a/nar00439-0373-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b8f/324317/fa3c09a9338a/nar00439-0373-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b8f/324317/fa3c09a9338a/nar00439-0373-a.jpg

相似文献

1
Solid-phase synthesis of polynucleotides. IV. Usage of polystyrene resins for the synthesis of polydeoxyribonucleotides by the phosphostriester method.多核苷酸的固相合成。IV. 磷酸三酯法中聚苯乙烯树脂在聚脱氧核糖核苷酸合成中的应用。
Nucleic Acids Res. 1980 Nov 25;8(22):5507-17. doi: 10.1093/nar/8.22.5507.
2
Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method.多核苷酸的固相合成。III. 用片段偶联磷酸三酯法合成具有特定序列的多核苷酸。
Nucleic Acids Res. 1980 Nov 25;8(22):5491-505. doi: 10.1093/nar/8.22.5491.
3
Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.多核苷酸的固相合成。VI. 关于用于固相载体的聚苯乙烯共聚物的进一步研究。
Nucleic Acids Res. 1982 Mar 11;10(5):1755-69. doi: 10.1093/nar/10.5.1755.
4
Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method.多核苷酸的固相合成。II. 用片段偶联磷酸三酯法合成聚胸腺嘧啶核苷酸
Nucleic Acids Res. 1980 Nov 25;8(22):5473-89. doi: 10.1093/nar/8.22.5473.
5
Investigating resins for solid phase organic synthesis: the relationship between swelling and microenvironment as probed by EPR and fluorescence spectroscopy.用于固相有机合成的树脂研究：通过电子顺磁共振和荧光光谱法探究溶胀与微环境之间的关系。
J Comb Chem. 2000 Jul-Aug;2(4):330-6. doi: 10.1021/cc000007c.
6
4-Chloromethylphenoxyacetyl polystyrene and polyamide supports for solid-phase peptide synthesis.用于固相肽合成的4-氯甲基苯氧基乙酰基聚苯乙烯和聚酰胺载体。
Int J Pept Protein Res. 1983 Feb;21(2):118-26. doi: 10.1111/j.1399-3011.1983.tb03085.x.
7
Synthesis and physicochemical properties of two analogs of poly(dA): poly(2-aminopurine-9-beta-D-deoxyribonucleotide) and poly 2-amino-deoxyadenylic acid.聚（dA）的两种类似物的合成及物理化学性质：聚（2-氨基嘌呤-9-β-D-脱氧核糖核苷酸）和聚2-氨基脱氧腺苷酸。
Nucleic Acids Res. 1982 Jul 10;10(13):4059-69. doi: 10.1093/nar/10.13.4059.
8
Polynucleotides. LVI. Synthesis and properties of poly(2-deoxy-2'-fluoroinosinic acid).多核苷酸。LVI。聚（2-脱氧-2'-氟肌苷酸）的合成与性质
Nucleic Acids Res. 1978 Sep;5(9):3315-24. doi: 10.1093/nar/5.9.3315.
9
A new condensing reagent, 1-(2,4,6-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide and its use in the synthesis of lambda cro binding heptadecanucleotide on a polymer support.一种新型缩合试剂，1-(2,4,6-三异丙基苯磺酰基)-5-(吡啶-2-基)四唑化物及其在聚合物载体上合成λ cro结合十七聚核苷酸中的应用。
Nucleic Acids Res. 1982 Oct 25;10(20):6235-41. doi: 10.1093/nar/10.20.6235.
10
Syntheses, characterization and application of cross-linked polystyrene-ethyleneglycol acrylate resin (CLPSER) as a novel polymer support for polypeptide syntheses.交联聚苯乙烯-乙二醇丙烯酸酯树脂（CLPSER）作为一种用于多肽合成的新型聚合物载体的合成、表征及应用
J Pept Res. 2001 Aug;58(2):117-28. doi: 10.1034/j.1399-3011.2001.00877.x.

引用本文的文献

1
Prolipoprotein signal peptidase of Escherichia coli requires a cysteine residue at the cleavage site.大肠杆菌的前脂蛋白信号肽酶在切割位点需要一个半胱氨酸残基。
EMBO J. 1983;2(1):87-91. doi: 10.1002/j.1460-2075.1983.tb01386.x.
2
Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.寡脱氧核糖核苷酸的快速合成VI。通过改进的固相磷三酯路线高效、机械化合成十七聚脱氧核糖核苷酸。
Nucleic Acids Res. 1981 Apr 10;9(7):1691-706. doi: 10.1093/nar/9.7.1691.
3
New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.

本文引用的文献

1
DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.DNA序列分析：一种通过图谱对大的寡脱氧核糖核苷酸片段进行测序的通用、简单且快速的方法。
Nucleic Acids Res. 1974 Mar;1(3):331-53. doi: 10.1093/nar/1.3.331.
2
Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method.多核苷酸的固相合成。III. 用片段偶联磷酸三酯法合成具有特定序列的多核苷酸。
Nucleic Acids Res. 1980 Nov 25;8(22):5491-505. doi: 10.1093/nar/8.22.5491.
3
Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method.
通过磷酸三酯中间体合成寡核苷酸的新有效方法。
Nucleic Acids Res. 1982 Nov 11;10(21):6675-94. doi: 10.1093/nar/10.21.6675.
4
Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotides.脱氧寡核苷酸固相磷三酯合成法的改进
Nucleic Acids Res. 1982 Sep 25;10(18):5605-20. doi: 10.1093/nar/10.18.5605.
5
Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.多核苷酸的固相合成。VI. 关于用于固相载体的聚苯乙烯共聚物的进一步研究。
Nucleic Acids Res. 1982 Mar 11;10(5):1755-69. doi: 10.1093/nar/10.5.1755.
6
Role of positive charge on the amino-terminal region of the signal peptide in protein secretion across the membrane.信号肽氨基末端区域的正电荷在蛋白质跨膜分泌中的作用。
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3438-41. doi: 10.1073/pnas.79.11.3438.
7
Cloning and sequence determination of the gene for the human immunoglobulin epsilon chain expressed in a myeloma cell line.在骨髓瘤细胞系中表达的人免疫球蛋白ε链基因的克隆与序列测定。
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6661-5. doi: 10.1073/pnas.79.21.6661.
8
Recent developments in the chemical synthesis of polynucleotides.多核苷酸化学合成的最新进展。
Nucleic Acids Res. 1982 Nov 11;10(21):6553-70. doi: 10.1093/nar/10.21.6553.
9
Molecular cloning and sequence determination of rat preproenkephalin cDNA: sensitive probe for studying transcriptional changes in rat tissues.大鼠前脑啡肽原cDNA的分子克隆与序列测定：用于研究大鼠组织转录变化的灵敏探针。
Proc Natl Acad Sci U S A. 1984 Dec;81(23):7651-5. doi: 10.1073/pnas.81.23.7651.
10
Computer programs in nucleic acid synthesis: synthetic strategy development using solid-phase chemical techniques with data storage, retrieval and analysis capabilities.核酸合成中的计算机程序：利用具有数据存储、检索和分析能力的固相化学技术进行合成策略开发。
Nucleic Acids Res. 1984 Mar 12;12(5):2581-91. doi: 10.1093/nar/12.5.2581.
多核苷酸的固相合成。II. 用片段偶联磷酸三酯法合成聚胸腺嘧啶核苷酸
Nucleic Acids Res. 1980 Nov 25;8(22):5473-89. doi: 10.1093/nar/8.22.5473.
4
The monitoring of reactions in solid-phase peptide synthesis with picric acid.用苦味酸监测固相肽合成中的反应。
Anal Chim Acta. 1972 Jan;58(1):248-9. doi: 10.1016/S0003-2670(00)86882-8.
5
Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method.寡脱氧核糖核苷酸的快速合成：一种新的固相方法。
Nucleic Acids Res. 1977 Apr;4(4):1135-58. doi: 10.1093/nar/4.4.1135.
6
Rapid synthesis of oligodeoxyribonucleotides on a grafted polymer support.在接枝聚合物载体上快速合成寡脱氧核糖核苷酸。
Nucleic Acids Res. 1979;6(6):2041-56. doi: 10.1093/nar/6.6.2041.
7
Tert-butoxycarbonylaminoacyl-4-(oxymethyl)-phenylacetamidomethyl-resin, a more acid-resistant support for solid-phase peptide synthesis.叔丁氧羰基氨基酰基-4-（氧甲基）-苯基乙酰氨基甲基树脂，一种用于固相肽合成的更耐酸的载体。
J Am Chem Soc. 1976 Nov 10;98(23):7357-62. doi: 10.1021/ja00439a041.